Detailed information of TA system TA142916

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3382574..3383231
• Replicon: chromosome
• Accession: NZ_CP045868
• Organism: Klebsiella aerogenes strain Y6

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A0H3FM52
• Locus tag: GIY02_RS16355
• Protein ID: WP_015369792.1
• Coordinates: 3382821..3383231 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: GIY02_RS16350
• Protein ID: WP_032709107.1
• Coordinates: 3382574..3382840 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY02_RS16325	3377801..3379234	-	1434	WP_032709109.1	6-phospho-beta-glucosidase BglA	-
GIY02_RS16330	3379354..3380082	-	729	WP_015369788.1	MurR/RpiR family transcriptional regulator	-
GIY02_RS16335	3380133..3380444	+	312	WP_015703422.1	N(4)-acetylcytidine aminohydrolase	-
GIY02_RS16340	3380609..3381268	+	660	WP_008806429.1	hemolysin III family protein	-
GIY02_RS16345	3381367..3382350	-	984	WP_032709108.1	tRNA-modifying protein YgfZ	-
GIY02_RS16350	3382574..3382840	+	267	WP_032709107.1	FAD assembly factor SdhE	Antitoxin
GIY02_RS16355	3382821..3383231	+	411	WP_015369792.1	protein YgfX	Toxin
GIY02_RS16360	3383239..3383760	-	522	WP_015369793.1	flavodoxin FldB	-
GIY02_RS16365	3383861..3384757	+	897	WP_015703420.1	site-specific tyrosine recombinase XerD	-
GIY02_RS16370	3384780..3385493	+	714	WP_015369795.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GIY02_RS16375	3385499..3387232	+	1734	WP_015369796.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15887.69 Da
• Isoelectric Point: 10.0200

>T142916 WP_015369792.1 NZ_CP045868:3382821-3383231 [Klebsiella aerogenes]
VVLWQSDLRISWRSQWFSLLLHGVVAAIVLLMPWPLSYTPIWLLLLSLVVFDCVRSQRRIHACQGEIKLLIDSRLRWQKT
EWDIVGTPWVISSGMLLRLKHAETGRSQHLWVAADSMDAGEWRDLRRLVLQKPAHD

Nucleotide

• Length: 411 bp

>T142916 NZ_CP060902:1352296-1352694 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10609.12 Da
• Isoelectric Point: 5.6963

>AT142916 WP_032709107.1 NZ_CP045868:3382574-3382840 [Klebsiella aerogenes]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFIRLLENDDPDLFNWLMNHGKPADTQLQRMINLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT142916 NZ_CP060902:1352069-1352296 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A0H3FM52