Detailed information of TA system TA142915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2864622..2865208
• Replicon: chromosome
• Accession: NZ_CP045868
• Organism: Klebsiella aerogenes strain Y6

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GIY02_RS13785
• Protein ID: WP_032709318.1
• Coordinates: 2864840..2865208 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: GIY02_RS13780
• Protein ID: WP_004174006.1
• Coordinates: 2864622..2864843 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY02_RS13760	2860763..2861689	+	927	WP_015369323.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
GIY02_RS13765	2861686..2862963	+	1278	WP_015369324.1	branched chain amino acid ABC transporter permease LivM	-
GIY02_RS13770	2862960..2863727	+	768	WP_032709319.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
GIY02_RS13775	2863745..2864458	+	714	WP_086538103.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
GIY02_RS13780	2864622..2864843	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GIY02_RS13785	2864840..2865208	+	369	WP_032709318.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GIY02_RS13790	2865500..2866816	+	1317	WP_015703703.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
GIY02_RS13795	2866923..2867810	+	888	WP_015369328.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
GIY02_RS13800	2867807..2868652	+	846	WP_015369329.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
GIY02_RS13805	2868654..2869724	+	1071	WP_032709316.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2860763..2870461	9698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.97 Da
• Isoelectric Point: 9.7753

>T142915 WP_032709318.1 NZ_CP045868:2864840-2865208 [Klebsiella aerogenes]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGKLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T142915 NZ_CP060902:1119246-1119557 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT142915 WP_004174006.1 NZ_CP045868:2864622-2864843 [Klebsiella aerogenes]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT142915 NZ_CP060902:1119554-1119973 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7