Detailed information of TA system TA142904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1328987..1329516
• Replicon: chromosome
• Accession: NZ_CP045866
• Organism: Staphylococcus aureus strain CFSAN007894

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AB466_RS06665
• Protein ID: WP_000621175.1
• Coordinates: 1328987..1329349 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AB466_RS06670
• Protein ID: WP_000948331.1
• Coordinates: 1329346..1329516 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB466_RS06645	1325965..1326735	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
AB466_RS06650	1326710..1327189	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AB466_RS06655	1327191..1327517	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AB466_RS06660	1327636..1328637	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AB466_RS06665	1328987..1329349	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB466_RS06670	1329346..1329516	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AB466_RS06675	1329601..1330749	-	1149	WP_001281154.1	alanine racemase	-
AB466_RS06680	1330815..1331174	-	360	WP_000581197.1	holo-ACP synthase	-
AB466_RS06685	1331178..1331669	-	492	WP_001205912.1	PH domain-containing protein	-
AB466_RS06690	1331656..1333239	-	1584	WP_001294626.1	PH domain-containing protein	-
AB466_RS06695	1333232..1333711	-	480	WP_001287087.1	hypothetical protein	-
AB466_RS06700	1333919..1334479	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T142904 WP_000621175.1 NZ_CP045866:c1329349-1328987 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T142904 NZ_CP060899:c3980874-3980587 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT142904 WP_000948331.1 NZ_CP045866:c1329516-1329346 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT142904 NZ_CP060899:c3980600-3980298 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCAATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1