Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 87320..87879 Replicon plasmid p1CFSAN076619
Accession NZ_CP045864
Organism Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag GIX96_RS26620 Protein ID WP_000421248.1
Coordinates 87320..87595 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag GIX96_RS26625 Protein ID WP_001178089.1
Coordinates 87595..87879 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIX96_RS26585 82673..83062 - 390 WP_001172748.1 cytochrome b562 family protein -
GIX96_RS26590 83106..85316 - 2211 WP_000592771.1 catalase/peroxidase KatP -
GIX96_RS26595 85493..85678 + 186 WP_001302179.1 hypothetical protein -
GIX96_RS26600 85678..86094 + 417 Protein_82 IS91 family transposase -
GIX96_RS26605 86094..86660 + 567 Protein_83 IS3-like element IS600 family transposase -
GIX96_RS26610 86704..86919 + 216 Protein_84 transposase -
GIX96_RS26615 87019..87225 - 207 WP_001105064.1 hypothetical protein -
GIX96_RS26620 87320..87595 - 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GIX96_RS26625 87595..87879 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
GIX96_RS26630 88792..89649 - 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
GIX96_RS26635 89642..89716 - 75 WP_001370046.1 RepA leader peptide Tap -
GIX96_RS26640 89952..90206 - 255 WP_000083831.1 replication regulatory protein RepA -
GIX96_RS26645 90446..90784 - 339 WP_000766796.1 hypothetical protein -
GIX96_RS26650 90822..91031 - 210 WP_001302200.1 hemolysin expression modulator Hha -
GIX96_RS26655 91077..91538 - 462 WP_001233853.1 thermonuclease family protein -
GIX96_RS26660 91783..91995 - 213 WP_001302189.1 ANR family transcriptional regulator -
GIX96_RS26665 92128..92688 - 561 WP_000840472.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP 1..95989 95989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T142895 WP_000421248.1 NZ_CP045864:c87595-87320 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T142895 NZ_CP060899:2286416-2287738 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT142895 WP_001178089.1 NZ_CP045864:c87879-87595 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT142895 NZ_CP060899:2286150-2286416 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References