Detailed information of TA system TA142893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5166585..5167135
• Replicon: chromosome
• Accession: NZ_CP045863
• Organism: Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: GIX96_RS25420
• Protein ID: WP_001160975.1
• Coordinates: 5166821..5167135 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: GIX96_RS25415
• Protein ID: WP_000125566.1
• Coordinates: 5166585..5166818 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIX96_RS25390	5161659..5161946	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
GIX96_RS25395	5162004..5163335	+	1332	WP_001183177.1	MFS transporter	-
GIX96_RS25400	5163443..5163973	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
GIX96_RS25405	5163966..5165828	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
GIX96_RS25410	5166020..5166499	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
GIX96_RS25415	5166585..5166818	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GIX96_RS25420	5166821..5167135	+	315	WP_001160975.1	CcdB family protein	Toxin
GIX96_RS25425	5167132..5167980	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
GIX96_RS25430	5167987..5168364	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
GIX96_RS25435	5168367..5169188	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
GIX96_RS25440	5169185..5170174	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GIX96_RS25445	5170174..5171460	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T142893 WP_001160975.1 NZ_CP045863:5166821-5167135 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T142893 NZ_CP060899:1777153-1777407 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT142893 WP_000125566.1 NZ_CP045863:5166585-5166818 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT142893 NZ_CP060899:1776905-1777156 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9