Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4021694..4022325 Replicon chromosome
Accession NZ_CP045863
Organism Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag GIX96_RS20245 Protein ID WP_001259386.1
Coordinates 4022050..4022325 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GIX96_RS20240 Protein ID WP_000593555.1
Coordinates 4021694..4022053 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIX96_RS20210 4016806..4017279 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
GIX96_RS20215 4017276..4017557 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
GIX96_RS20220 4017634..4018992 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
GIX96_RS20225 4018985..4020493 + 1509 WP_001301773.1 carbohydrate kinase -
GIX96_RS20230 4020483..4020752 + 270 WP_000933060.1 HPr family phosphocarrier protein -
GIX96_RS20235 4020784..4021644 + 861 WP_001131788.1 class II aldolase -
GIX96_RS20240 4021694..4022053 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GIX96_RS20245 4022050..4022325 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
GIX96_RS20250 4022398..4023522 - 1125 WP_001301659.1 ABC transporter permease -
GIX96_RS20255 4023522..4026257 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
GIX96_RS20260 4026254..4027321 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T142888 WP_001259386.1 NZ_CP045863:c4022325-4022050 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T142888 NZ_CP060899:c593892-593428 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT142888 WP_000593555.1 NZ_CP045863:c4022053-4021694 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT142888 NZ_CP060899:c594227-593892 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References