Detailed information of TA system TA142885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3930211..3930965
• Replicon: chromosome
• Accession: NZ_CP045863
• Organism: Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: GIX96_RS19775
• Protein ID: WP_001301452.1
• Coordinates: 3930480..3930965 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: GIX96_RS19770
• Protein ID: WP_000801912.1
• Coordinates: 3930211..3930489 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIX96_RS19765	3927439..3930144	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
GIX96_RS19770	3930211..3930489	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
GIX96_RS19775	3930480..3930965	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
GIX96_RS19780	3931015..3932043	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
GIX96_RS19785	3932047..3933273	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
GIX96_RS19790	3933461..3935059	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
GIX96_RS19795	3935041..3935799	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T142885 WP_001301452.1 NZ_CP045863:3930480-3930965 [Escherichia coli O157:H7]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T142885 NZ_CP060894:31111-31386 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT142885 WP_000801912.1 NZ_CP045863:3930211-3930489 [Escherichia coli O157:H7]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT142885 NZ_CP060894:30827-31111 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9