Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1690591..1691229 Replicon chromosome
Accession NZ_CP045863
Organism Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GIX96_RS08425 Protein ID WP_000813794.1
Coordinates 1690591..1690767 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GIX96_RS08430 Protein ID WP_001270286.1
Coordinates 1690813..1691229 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIX96_RS08405 1686213..1687424 - 1212 WP_071525382.1 BenE family transporter YdcO -
GIX96_RS08410 1687477..1688013 + 537 WP_000429134.1 DNA-binding transcriptional regulator SutR -
GIX96_RS08415 1688086..1690047 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GIX96_RS08420 1690139..1690369 - 231 WP_000494244.1 YncJ family protein -
GIX96_RS08425 1690591..1690767 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GIX96_RS08430 1690813..1691229 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GIX96_RS08435 1691308..1692714 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
GIX96_RS08440 1692959..1694104 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
GIX96_RS08445 1694122..1695135 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
GIX96_RS08450 1695136..1696077 + 942 WP_001251320.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T142871 WP_000813794.1 NZ_CP045863:1690591-1690767 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T142871 NZ_CP060892:1886970-1887224 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT142871 WP_001270286.1 NZ_CP045863:1690813-1691229 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT142871 NZ_CP060892:1886722-1886973 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References