Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 319449..320067 Replicon chromosome
Accession NZ_CP045863
Organism Escherichia coli O157:H7 strain ATCC 43890

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GIX96_RS01550 Protein ID WP_001291435.1
Coordinates 319449..319667 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GIX96_RS01555 Protein ID WP_000344800.1
Coordinates 319693..320067 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIX96_RS01515 314736..315308 + 573 WP_000779831.1 YbaY family lipoprotein -
GIX96_RS01520 315341..315652 - 312 WP_000409911.1 MGMT family protein -
GIX96_RS01530 316031..316384 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GIX96_RS01535 316426..317976 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GIX96_RS01540 318140..318610 - 471 WP_000136192.1 YlaC family protein -
GIX96_RS01545 318726..319277 - 552 WP_000102553.1 maltose O-acetyltransferase -
GIX96_RS01550 319449..319667 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GIX96_RS01555 319693..320067 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GIX96_RS01560 320613..323762 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
GIX96_RS01565 323785..324978 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T142863 WP_001291435.1 NZ_CP045863:c319667-319449 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T142863 NZ_CP060892:186419-186526 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT142863 WP_000344800.1 NZ_CP045863:c320067-319693 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT142863 NZ_CP060892:c186371-186305 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References