Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/RHH(antitoxin)
Location 2667507..2668060 Replicon chromosome
Accession NZ_CP045858
Organism Pseudomonas balearica strain EC28

Toxin (Protein)


Gene name parE Uniprot ID Q88JZ3
Locus tag GII23_RS12765 Protein ID WP_010953434.1
Coordinates 2667767..2668060 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2W5CWX7
Locus tag GII23_RS12760 Protein ID WP_017940300.1
Coordinates 2667507..2667779 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII23_RS12735 2662571..2663173 + 603 WP_165563271.1 reverse transcriptase N-terminal domain-containing protein -
GII23_RS12740 2663328..2664131 - 804 WP_013983804.1 IS21-like element ISPst3 family helper ATPase IstB -
GII23_RS12745 2664124..2665623 - 1500 WP_165564135.1 IS21-like element ISPst3 family transposase -
GII23_RS12750 2666018..2666401 - 384 Protein_2502 transposase -
GII23_RS12755 2666573..2666812 - 240 WP_165564161.1 AlpA family phage regulatory protein -
GII23_RS12760 2667507..2667779 + 273 WP_017940300.1 CopG family ribbon-helix-helix protein Antitoxin
GII23_RS12765 2667767..2668060 + 294 WP_010953434.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GII23_RS12770 2668054..2669463 - 1410 WP_165563272.1 site-specific integrase -
GII23_RS12775 2669792..2670466 - 675 Protein_2507 tyrosine-type recombinase/integrase -
GII23_RS12780 2670765..2670911 - 147 WP_165563273.1 transposase family protein -
GII23_RS21860 2671234..2671394 - 161 Protein_2509 helix-turn-helix domain-containing protein -
GII23_RS12785 2671581..2672804 - 1224 Protein_2510 NADH-quinone oxidoreductase subunit N -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2639419..2670454 31035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-95)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11155.61 Da        Isoelectric Point: 5.9081

>T142858 WP_010953434.1 NZ_CP045858:2667767-2668060 [Pseudomonas balearica]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Download         Length: 294 bp

>T142858 NZ_CP060885:c4365710-4365537 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10327.45 Da        Isoelectric Point: 5.5751

>AT142858 WP_017940300.1 NZ_CP045858:2667507-2667779 [Pseudomonas balearica]
MATSIKIDDDLKGRIQHLAGLRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGQHLTGQEARTWLGSWGTD
AEAELPKCHD

Download         Length: 273 bp

>AT142858 NZ_CP060885:c4366034-4365738 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W5CNE8

Antitoxin

Source ID Structure
AlphaFold DB A0A2W5CWX7

References