Detailed information of TA system TA142857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prcAT/RES-TIGR02293
• Location: 2614618..2615501
• Replicon: chromosome
• Accession: NZ_CP045858
• Organism: Pseudomonas balearica strain EC28

Toxin (Protein)

• Gene name: PrcT
• Uniprot ID: S6AYH8
• Locus tag: GII23_RS12500
• Protein ID: WP_011078034.1
• Coordinates: 2615064..2615501 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: PrcA
• Uniprot ID: S6AKX1
• Locus tag: GII23_RS12495
• Protein ID: WP_011078033.1
• Coordinates: 2614618..2615067 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII23_RS12465	2610050..2611303	-	1254	WP_009238019.1	MgtC/SapB family protein	-
GII23_RS12470	2611479..2611685	-	207	WP_024542659.1	hypothetical protein	-
GII23_RS12475	2611764..2613281	+	1518	Protein_2448	Tn3 family transposase	-
GII23_RS12480	2613310..2613642	-	333	Protein_2449	transposase	-
GII23_RS12485	2613756..2613878	+	123	WP_165564159.1	DUF1289 domain-containing protein	-
GII23_RS12490	2614002..2614427	+	426	WP_165563260.1	DUF4399 domain-containing protein	-
GII23_RS12495	2614618..2615067	+	450	WP_011078033.1	DUF2384 domain-containing protein	Antitoxin
GII23_RS12500	2615064..2615501	+	438	WP_011078034.1	RES family NAD+ phosphorylase	Toxin
GII23_RS12505	2615528..2615788	+	261	Protein_2454	endonuclease	-
GII23_RS12510	2615830..2616759	-	930	WP_003150544.1	recombinase family protein	-
GII23_RS12520	2616976..2617359	+	384	Protein_2456	transposase	-
GII23_RS12525	2617648..2618883	-	1236	WP_003089075.1	TolC family protein	-
GII23_RS12530	2618883..2619185	-	303	WP_003288512.1	DUF3240 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2568570..2616759	48189
	-	inside	IScluster/Tn	-	-	2605665..2617359	11694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-134)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (97-146)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15867.09 Da
• Isoelectric Point: 4.4777

>T142857 WP_011078034.1 NZ_CP045858:2615064-2615501 [Pseudomonas balearica]
VILWRISSYADLSGTGGLRVSGRWHQAGRPVVYTATSPPGAMLEVLVHLEIDPEDFPATMQLLRIEVPDSVSQAQMPTLP
AGWSAETELTRGIGNQFLDECNALLLPVPSAIIPSTVNYLFNPRHPEAQSATIQVELFTPDSRLF

Nucleotide

• Length: 438 bp

>T142857 NZ_CP060885:3586833-3587051 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16963.45 Da
• Isoelectric Point: 6.1074

>AT142857 WP_011078033.1 NZ_CP045858:2614618-2615067 [Pseudomonas balearica]
MLAEVLRDHGYSAYRARLQILLEIPESASDFEIHTRISHGFSAARLMRLTEEGVVTPLERDQIIPLRTLKNRIERDQPLT
VEESDRLFRSAHITAMAEAIFGDADKAKRWLSKPKERFSGLTPMQLLTTQQGTSQVEEMLLQIAEGFAL

Nucleotide

• Length: 450 bp

>AT142857 NZ_CP060885:3586433-3586807 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: S6AYH8

Antitoxin

• AlphaFold DB: S6AKX1