Detailed information of TA system TA142852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 146962..148448
• Replicon: plasmid punanmed2
• Accession: NZ_CP045856
• Organism: Agrobacterium sp. MA01

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A1B3P5E9
• Locus tag: GH983_RS23355
• Protein ID: WP_069044144.1
• Coordinates: 147186..148448 (+)
• Length: 421 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: GH983_RS23350
• Protein ID: WP_083229771.1
• Coordinates: 146962..147189 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH983_RS23330	142081..143520	+	1440	WP_069044142.1	aldehyde dehydrogenase family protein	-
GH983_RS23335	143701..144162	+	462	Protein_139	transposase	-
GH983_RS23340	144448..145419	+	972	WP_083229798.1	NAD(P)H-binding protein	-
GH983_RS23345	145555..146478	+	924	WP_153763921.1	alpha/beta hydrolase	-
GH983_RS23350	146962..147189	+	228	WP_083229771.1	helix-turn-helix transcriptional regulator	Antitoxin
GH983_RS23355	147186..148448	+	1263	WP_069044144.1	type II toxin-antitoxin system HipA family toxin	Toxin
GH983_RS23360	148657..150459	-	1803	WP_069044145.1	NAD(P)/FAD-dependent oxidoreductase	-
GH983_RS23365	150578..151651	-	1074	WP_069044146.1	2,3-butanediol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	tviB / bplB / bplC	1..310891	310891
	-	flank	IS/Tn	-	-	143701..144219	518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-370)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-48)

Sequences

Toxin

Protein

• Length: 421 a.a.
• Molecular weight: 45830.71 Da
• Isoelectric Point: 5.5201

>T142852 WP_069044144.1 NZ_CP045856:147186-148448 [Agrobacterium sp. MA01]
MTRVLDVYFRDTKAGVLGRLDDGLLTFAYDGAYLLDQRSRAISFSMPLQEKPFGDQVVRPFFSGLLPDEGARQRLAGALG
LSAGNAFGLLEIIGGECAGALSLYPPGTAPEPPGQSQAEILTSERLDEILSRLRTRPLLGGDEGIRLSLAGAQDKLAVIV
DGDSIALARDGRPTTHILKPLIQALEGTVENEYFCMRLGARLKLPVPHVEIRLDGATAFLLVERYDRTKAADGIIERLHQ
EDFCQALSVPPELKYEAEGGPGTERALGLIDQVCARPAADRLQFIRMLIFHYLVGNADAHGKNYALLYGSDEPDLAPIYD
VVCTAVYPRLSKRLAMKIGGRDVPDTIQMKHWTTLVPETKSSQRILVRDLAQMAERILSEADALVAELADEGIRHPILSA
IRKVISTRAGLLQKAAEQVS

Nucleotide

• Length: 1263 bp

>T142852 NZ_CP060885:c1877925-1877551 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8151.52 Da
• Isoelectric Point: 10.7429

>AT142852 WP_083229771.1 NZ_CP045856:146962-147189 [Agrobacterium sp. MA01]
METIKDTKSLGMLIREERKQQGLTQEQLAGVIGVGVRFVRELEAGKESCQIGRAMQVLAGLGLVVSVGRRQGPRS

Nucleotide

• Length: 228 bp

>AT142852 NZ_CP060885:c1878383-1878015 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1B3P5E9