Detailed information of TA system TA142845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 963220..963773
• Replicon: chromosome
• Accession: NZ_CP045854
• Organism: Agrobacterium sp. MA01

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GH983_RS04580
• Protein ID: WP_153761754.1
• Coordinates: 963528..963773 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GH983_RS04575
• Protein ID: WP_153761753.1
• Coordinates: 963220..963531 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH983_RS04535	958273..958500	-	228	WP_006727163.1	hypothetical protein	-
GH983_RS04540	958633..958869	-	237	WP_054151125.1	30S ribosomal protein S21	-
GH983_RS04545	959110..959319	-	210	WP_006727165.1	cold-shock protein	-
GH983_RS04550	959332..959676	-	345	WP_069041869.1	hypothetical protein	-
GH983_RS04555	959782..960273	+	492	WP_054151123.1	hypothetical protein	-
GH983_RS04560	960589..961638	+	1050	WP_069041868.1	fatty acid desaturase	-
GH983_RS04565	961732..962427	-	696	WP_153761751.1	YafY family transcriptional regulator	-
GH983_RS04570	962552..963187	+	636	WP_153761752.1	LysE family translocator	-
GH983_RS04575	963220..963531	-	312	WP_153761753.1	BrnA antitoxin family protein	Antitoxin
GH983_RS04580	963528..963773	-	246	WP_153761754.1	BrnT family toxin	Toxin
GH983_RS04585	963837..964157	-	321	WP_153761755.1	YnfA family protein	-
GH983_RS04590	964220..965038	-	819	WP_054151117.1	EAL domain-containing protein	-
GH983_RS04595	965180..966757	-	1578	WP_054151116.1	DUF853 domain-containing protein	-
GH983_RS04600	967002..967394	+	393	WP_153761756.1	DUF937 domain-containing protein	-
GH983_RS04605	967620..968096	+	477	WP_153761757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9058.48 Da
• Isoelectric Point: 10.9487

>T142845 WP_153761754.1 NZ_CP045854:c963773-963528 [Agrobacterium sp. MA01]
VIVWDEPKRQQNLAKHSLDFAELDLSFFLTASIRTAKTGRAIAVGEFQGRAIVAVVFLPMGREAISIISMRPASRRERSE
T

Nucleotide

• Length: 246 bp

>T142845 NZ_CP060885:777288-777584 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11724.32 Da
• Isoelectric Point: 5.3322

>AT142845 WP_153761753.1 NZ_CP045854:c963531-963220 [Agrobacterium sp. MA01]
MKNKFSSARPLTDEEEAEIQAMSASDPDNPEMTEEELKEFRPFREVYPDLAAAIDRKLGRPKAKTTKKAISLRLDQEVIE
RFKAGGEGWQSRMNEVLRKAVGL

Nucleotide

• Length: 312 bp

>AT142845 NZ_CP060885:777586-777981 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA