Detailed information of TA system TA142842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2424888..2425453
• Replicon: chromosome
• Accession: NZ_CP045847
• Organism: Streptomyces clavuligerus strain ATCC 27064 isolate Leon-isolate

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: GE265_RS09785
• Protein ID: WP_003960643.1
• Coordinates: 2424888..2425256 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: GE265_RS09790
• Protein ID: WP_029182939.1
• Coordinates: 2425256..2425453 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE265_RS09765	2420206..2420676	+	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-
GE265_RS09770	2420741..2421529	-	789	WP_003960641.1	hypothetical protein	-
GE265_RS09775	2421994..2422635	-	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
GE265_RS09780	2422937..2424730	-	1794	WP_003954793.1	DEAD/DEAH box helicase	-
GE265_RS09785	2424888..2425256	-	369	WP_003960643.1	Fic family protein	Toxin
GE265_RS09790	2425256..2425453	-	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
GE265_RS09795	2425487..2426992	-	1506	WP_003954790.1	MFS transporter	-
GE265_RS09805	2427503..2428066	-	564	WP_003954788.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T142842 WP_003960643.1 NZ_CP045847:c2425256-2424888 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T142842 NZ_CP060885:326830-327060 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT142842 WP_029182939.1 NZ_CP045847:c2425453-2425256 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT142842 NZ_CP060885:327057-327416 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: E2Q583