Detailed information of TA system TA142841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 85433..87056
• Replicon: plasmid pN2-1
• Accession: NZ_CP045846
• Organism: Kluyvera intermedia strain N2-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: GHC21_RS22185
• Protein ID: WP_000088046.1
• Coordinates: 85731..87056 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: GHC21_RS22180
• Protein ID: WP_024136327.1
• Coordinates: 85433..85726 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC21_RS22155	81305..82546	+	1242	WP_001053338.1	VWA domain-containing protein	-
GHC21_RS22160	83109..83390	-	282	WP_000077926.1	hypothetical protein	-
GHC21_RS22165	83440..83631	-	192	WP_000398480.1	hypothetical protein	-
GHC21_RS22170	83723..84064	-	342	WP_001151575.1	hypothetical protein	-
GHC21_RS22175	84437..84829	+	393	WP_000341066.1	hypothetical protein	-
GHC21_RS22180	85433..85726	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
GHC21_RS22185	85731..87056	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
GHC21_RS22190	87117..87323	+	207	WP_000134171.1	hypothetical protein	-
GHC21_RS22195	87425..87835	+	411	WP_000985911.1	hypothetical protein	-
GHC21_RS22200	87848..88663	+	816	WP_001572439.1	HNH endonuclease	-
GHC21_RS22205	88917..89342	+	426	WP_001043843.1	hypothetical protein	-
GHC21_RS22210	89796..90032	+	237	Protein_85	hypothetical protein	-
GHC21_RS22215	90087..90386	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / floR / blaCTX-M-65 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 / sul1	-	1..245785	245785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T142841 WP_000088046.1 NZ_CP045846:85731-87056 [Kluyvera intermedia]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T142841 NZ_CP060882:15498-15803 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT142841 WP_024136327.1 NZ_CP045846:85433-85726 [Kluyvera intermedia]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT142841 NZ_CP060882:15278-15496 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1