Detailed information of TA system TA142840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3447051..3447669
• Replicon: chromosome
• Accession: NZ_CP045845
• Organism: Kluyvera intermedia strain N2-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A447MKF0
• Locus tag: GHC21_RS16240
• Protein ID: WP_062773821.1
• Coordinates: 3447451..3447669 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GHC21_RS16235
• Protein ID: WP_153743488.1
• Coordinates: 3447051..3447425 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC21_RS16225	3442137..3443333	+	1197	WP_062773816.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
GHC21_RS16230	3443356..3446502	+	3147	WP_153743487.1	multidrug efflux RND transporter permease subunit AcrB	-
GHC21_RS16235	3447051..3447425	+	375	WP_153743488.1	Hha toxicity modulator TomB	Antitoxin
GHC21_RS16240	3447451..3447669	+	219	WP_062773821.1	hemolysin expression modulator Hha	Toxin
GHC21_RS16245	3447810..3448364	+	555	WP_062773823.1	maltose O-acetyltransferase	-
GHC21_RS16250	3448468..3448938	+	471	WP_153743489.1	hypothetical protein	-
GHC21_RS16255	3448913..3450358	-	1446	WP_153743490.1	PLP-dependent aminotransferase family protein	-
GHC21_RS16260	3450461..3451171	+	711	WP_153743491.1	GNAT family N-acetyltransferase	-
GHC21_RS16265	3451168..3451308	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
GHC21_RS16270	3451308..3451571	-	264	WP_052283831.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8624.01 Da
• Isoelectric Point: 8.9008

>T142840 WP_062773821.1 NZ_CP045845:3447451-3447669 [Kluyvera intermedia]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPTVWKFIR

Nucleotide

• Length: 219 bp

>T142840 NZ_CP060881:2050-2328 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTCGTG
TGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
GTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14325.07 Da
• Isoelectric Point: 4.8887

>AT142840 WP_153743488.1 NZ_CP045845:3447051-3447425 [Kluyvera intermedia]
MDEYSPKRHDIAQLKFLCETLYHDSLATLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYSEDNKLIAQIDEYL
DDTFMLFGNYGINSDDLQKWRKSGNKLFRCFVNASRANPVSLSC

Nucleotide

• Length: 375 bp

>AT142840 NZ_CP060881:1781-2050 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTTCCGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A447MKF0