Detailed information of TA system TA142816

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 116661..117027
• Replicon: chromosome
• Accession: NZ_CP045843
• Organism: Kluyvera intermedia strain HR2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GHC38_RS00590
• Protein ID: WP_153741889.1
• Coordinates: 116661..116885 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: GHC38_RS00595
• Protein ID: WP_153741890.1
• Coordinates: 116920..117027 (-)
• Length: 36 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC38_RS00575	112294..114573	-	2280	WP_153741886.1	fimbrial biogenesis outer membrane usher protein	-
GHC38_RS00580	114644..115330	-	687	WP_153741887.1	molecular chaperone	-
GHC38_RS00585	115344..115841	-	498	WP_153741888.1	spore coat U domain-containing protein	-
GHC38_RS00590	116661..116885	-	225	WP_153741889.1	TA system toxin CbtA family protein	Toxin
GHC38_RS00595	116920..117027	-	108	WP_153741890.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GHC38_RS00600	117070..117576	-	507	WP_153741891.1	hypothetical protein	-
GHC38_RS00605	117855..118349	-	495	WP_153741892.1	hypothetical protein	-
GHC38_RS00610	118342..119004	-	663	WP_153741893.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	116661..128787	12126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-73)

Antitoxin

• Antitoxin CbeA (8-34)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8533.94 Da
• Isoelectric Point: 10.8616

>T142816 WP_153741889.1 NZ_CP045843:c116885-116661 [Kluyvera intermedia]
MNTLPAINQRAVQAHIDARITLVDALNFIVEKYELVRTDRPELTLRELSPFITVIDILRTRKVTGLGTRNGYRR

Nucleotide

• Length: 225 bp

>T142816 NZ_CP060874:c88275-87970 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 36 a.a.
• Molecular weight: 4157.90 Da
• Isoelectric Point: 9.7016

>AT142816 WP_153741890.1 NZ_CP045843:c117027-116920 [Kluyvera intermedia]
MEIFVFVLIKNEMTHKTHTLGSRGYIYVAIYPTQR

Nucleotide

• Length: 108 bp

>AT142816 NZ_CP060874:c88495-88277 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA