Detailed information of TA system TA142808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2539192..2539928
• Replicon: chromosome
• Accession: NZ_CP045840
• Organism: Citrobacter sp. S40-1-2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GHC23_RS12115
• Protein ID: WP_079934035.1
• Coordinates: 2539446..2539928 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: GHC23_RS12110
• Protein ID: WP_008784769.1
• Coordinates: 2539192..2539458 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC23_RS12090	2534255..2535442	+	1188	WP_153753069.1	outer membrane protein transport protein	-
GHC23_RS12095	2535680..2536309	+	630	WP_008784772.1	winged helix-turn-helix transcriptional regulator	-
GHC23_RS12100	2536415..2536801	-	387	WP_111465586.1	hypothetical protein	-
GHC23_RS12105	2537056..2539038	+	1983	WP_153753071.1	MBL fold metallo-hydrolase	-
GHC23_RS12110	2539192..2539458	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
GHC23_RS12115	2539446..2539928	+	483	WP_079934035.1	GNAT family N-acetyltransferase	Toxin
GHC23_RS12120	2540182..2541258	+	1077	WP_161799729.1	hypothetical protein	-
GHC23_RS12125	2542033..2542968	+	936	WP_047359049.1	hypothetical protein	-
GHC23_RS12130	2543239..2543643	+	405	WP_153753073.1	hypothetical protein	-
GHC23_RS12135	2543640..2543975	+	336	WP_194841944.1	hypothetical protein	-
GHC23_RS12140	2543992..2544351	+	360	WP_045269654.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2536415..2546073	9658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17660.55 Da
• Isoelectric Point: 10.1016

>T142808 WP_079934035.1 NZ_CP045840:2539446-2539928 [Citrobacter sp. S40-1-2]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRKVAGFYSLATGSVNRTEVTGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALTDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T142808 NZ_CP060873:c1876704-1876330 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT142808 WP_008784769.1 NZ_CP045840:2539192-2539458 [Citrobacter sp. S40-1-2]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT142808 NZ_CP060873:c1877162-1876794 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA