Detailed information of TA system TA142798

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 925817..926517
• Replicon: chromosome
• Accession: NZ_CP045840
• Organism: Citrobacter sp. S40-1-2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7H4NX47
• Locus tag: GHC23_RS04530
• Protein ID: WP_001546951.1
• Coordinates: 926194..926517 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GHC23_RS04525
• Protein ID: WP_049116113.1
• Coordinates: 925817..926173 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC23_RS04495	921769..922662	+	894	WP_048215606.1	50S ribosome-binding GTPase	-
GHC23_RS04500	922947..923624	+	678	WP_048215607.1	hypothetical protein	-
GHC23_RS04505	923723..924544	+	822	WP_048215608.1	DUF945 domain-containing protein	-
GHC23_RS04510	924575..925018	+	444	WP_048215609.1	antirestriction protein	-
GHC23_RS04515	925031..925573	+	543	WP_048215610.1	DNA repair protein RadC	-
GHC23_RS04520	925570..925791	+	222	WP_047644100.1	DUF987 family protein	-
GHC23_RS04525	925817..926173	+	357	WP_049116113.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GHC23_RS04530	926194..926517	+	324	WP_001546951.1	TA system toxin CbtA family protein	Toxin
GHC23_RS04535	926759..927295	-	537	WP_032944886.1	GNAT family N-acetyltransferase	-
GHC23_RS04540	928111..930573	+	2463	WP_057067439.1	poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12496.55 Da
• Isoelectric Point: 7.1331

>T142798 WP_001546951.1 NZ_CP045840:926194-926517 [Citrobacter sp. S40-1-2]
MKFLPTTNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Nucleotide

• Length: 324 bp

>T142798 NZ_CP060873:325494-325724 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13083.78 Da
• Isoelectric Point: 6.2018

>AT142798 WP_049116113.1 NZ_CP045840:925817-926173 [Citrobacter sp. S40-1-2]
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELILTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 357 bp

>AT142798 NZ_CP060873:325721-326080 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A7H4NX47