Detailed information of TA system TA142796

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 686637..687316
• Replicon: chromosome
• Accession: NZ_CP045840
• Organism: Citrobacter sp. S40-1-2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: GHC23_RS03320
• Protein ID: WP_106929604.1
• Coordinates: 686975..687316 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: GHC23_RS03315
• Protein ID: WP_106929602.1
• Coordinates: 686637..686954 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC23_RS03270	682069..682890	+	822	WP_153752214.1	DUF945 domain-containing protein	-
GHC23_RS03275	683107..683808	+	702	WP_153752216.1	WYL domain-containing protein	-
GHC23_RS03280	683849..684085	+	237	WP_032181217.1	hypothetical protein	-
GHC23_RS03285	684085..684528	+	444	WP_153752218.1	hypothetical protein	-
GHC23_RS03290	684552..685019	+	468	WP_106929591.1	hypothetical protein	-
GHC23_RS03295	685096..685335	+	240	WP_106929593.1	DUF905 domain-containing protein	-
GHC23_RS03300	685437..685895	+	459	WP_106929595.1	antirestriction protein	-
GHC23_RS03305	685911..686387	+	477	WP_106929597.1	RadC family protein	-
GHC23_RS03310	686396..686617	+	222	WP_106929599.1	DUF987 domain-containing protein	-
GHC23_RS03315	686637..686954	+	318	WP_106929602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GHC23_RS03320	686975..687316	+	342	WP_106929604.1	TA system toxin CbtA family protein	Toxin
GHC23_RS03330	687679..688185	+	507	WP_008785849.1	G/U mismatch-specific DNA glycosylase	-
GHC23_RS03335	688231..690078	-	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-
GHC23_RS03340	690363..692108	-	1746	WP_003828467.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	660392..687316	26924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12925.98 Da
• Isoelectric Point: 9.1225

>T142796 WP_106929604.1 NZ_CP045840:686975-687316 [Citrobacter sp. S40-1-2]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLQQCRKNSVR

Nucleotide

• Length: 342 bp

>T142796 NZ_CP060869:2265-2540 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12039.63 Da
• Isoelectric Point: 7.0917

>AT142796 WP_106929602.1 NZ_CP045840:686637-686954 [Citrobacter sp. S40-1-2]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGRFSDAECRKLDETFPHFIRQMESMLATGEVSPQHAHCVTLYH
NDFTCEADTLGSCGYVYIAVYHTQR

Nucleotide

• Length: 318 bp

>AT142796 NZ_CP060869:1981-2265 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA