Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 46856..47378 Replicon plasmid pH12-2
Accession NZ_CP045839
Organism Citrobacter sp. H12-3-2

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag GHC22_RS24320 Protein ID WP_000220561.1
Coordinates 46856..47137 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag GHC22_RS24325 Protein ID WP_000121743.1
Coordinates 47127..47378 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHC22_RS24285 41892..43391 - 1500 Protein_46 Tn3 family transposase -
GHC22_RS24290 43477..44229 + 753 WP_000602738.1 IS6 family transposase -
GHC22_RS24295 44280..44645 - 366 Protein_48 AAC(3) family N-acetyltransferase -
GHC22_RS24300 44651..44842 - 192 Protein_49 hypothetical protein -
GHC22_RS24305 44962..45150 + 189 WP_000957857.1 hypothetical protein -
GHC22_RS24310 45160..46359 + 1200 WP_112901278.1 IS91 family transposase -
GHC22_RS24315 46525..46617 + 93 Protein_52 IS6 family transposase -
GHC22_RS24320 46856..47137 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GHC22_RS24325 47127..47378 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
GHC22_RS24330 48613..49452 + 840 WP_024129959.1 replication initiation protein -
GHC22_RS24335 49473..50315 + 843 WP_000435064.1 replication initiation protein -
GHC22_RS24340 50319..50765 + 447 WP_001074384.1 hypothetical protein -
GHC22_RS24345 50833..51255 + 423 WP_179292036.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA12 / aadA2 / qacE / sul1 / rmtB / blaTEM-1B / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / lnu(F) / qnrS1 - 1..84545 84545


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T142794 WP_000220561.1 NZ_CP045839:c47137-46856 [Citrobacter sp. H12-3-2]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T142794 NZ_CP060868:c133937-133539 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCTG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT142794 WP_000121743.1 NZ_CP045839:c47378-47127 [Citrobacter sp. H12-3-2]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT142794 NZ_CP060868:c134164-133937 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTATCGGACGAACCCGACTCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAACGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References