Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4774721..4775337 Replicon chromosome
Accession NZ_CP045837
Organism Citrobacter sp. H12-3-2

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag GHC22_RS22660 Protein ID WP_020996118.1
Coordinates 4774721..4775095 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag GHC22_RS22665 Protein ID WP_020996119.1
Coordinates 4775095..4775337 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHC22_RS22645 4772224..4773126 + 903 WP_003825273.1 formate dehydrogenase O subunit beta -
GHC22_RS22650 4773123..4773758 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
GHC22_RS22655 4773755..4774684 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
GHC22_RS22660 4774721..4775095 - 375 WP_020996118.1 type II toxin-antitoxin system VapC family toxin Toxin
GHC22_RS22665 4775095..4775337 - 243 WP_020996119.1 ribbon-helix-helix domain-containing protein Antitoxin
GHC22_RS22670 4775543..4776472 + 930 WP_103285937.1 alpha/beta hydrolase -
GHC22_RS22675 4776557..4776868 + 312 WP_048215202.1 type II toxin-antitoxin system HigB family toxin -
GHC22_RS22680 4776865..4777311 + 447 WP_048215203.1 helix-turn-helix domain-containing protein -
GHC22_RS22685 4777329..4778270 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
GHC22_RS22690 4778315..4778752 - 438 WP_003825289.1 D-tyrosyl-tRNA(Tyr) deacylase -
GHC22_RS22695 4778749..4779621 - 873 WP_008786998.1 virulence factor BrkB family protein -
GHC22_RS22700 4779615..4780214 - 600 WP_153753923.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13648.86 Da        Isoelectric Point: 9.6927

>T142792 WP_020996118.1 NZ_CP045837:c4775095-4774721 [Citrobacter sp. H12-3-2]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEHGMKLPDAIILATAQIHRLTLVTRNTKDFAGISGVVTPYTL

Download         Length: 375 bp

>T142792 NZ_CP060868:c70248-69832 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9479.80 Da        Isoelectric Point: 4.3437

>AT142792 WP_020996119.1 NZ_CP045837:c4775337-4775095 [Citrobacter sp. H12-3-2]
MMVGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT142792 NZ_CP060868:c70475-70245 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References