Detailed information of TA system TA142792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4774721..4775337
• Replicon: chromosome
• Accession: NZ_CP045837
• Organism: Citrobacter sp. H12-3-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GHC22_RS22660
• Protein ID: WP_020996118.1
• Coordinates: 4774721..4775095 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GHC22_RS22665
• Protein ID: WP_020996119.1
• Coordinates: 4775095..4775337 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC22_RS22645	4772224..4773126	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
GHC22_RS22650	4773123..4773758	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
GHC22_RS22655	4773755..4774684	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
GHC22_RS22660	4774721..4775095	-	375	WP_020996118.1	type II toxin-antitoxin system VapC family toxin	Toxin
GHC22_RS22665	4775095..4775337	-	243	WP_020996119.1	ribbon-helix-helix domain-containing protein	Antitoxin
GHC22_RS22670	4775543..4776472	+	930	WP_103285937.1	alpha/beta hydrolase	-
GHC22_RS22675	4776557..4776868	+	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	-
GHC22_RS22680	4776865..4777311	+	447	WP_048215203.1	helix-turn-helix domain-containing protein	-
GHC22_RS22685	4777329..4778270	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
GHC22_RS22690	4778315..4778752	-	438	WP_003825289.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GHC22_RS22695	4778749..4779621	-	873	WP_008786998.1	virulence factor BrkB family protein	-
GHC22_RS22700	4779615..4780214	-	600	WP_153753923.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13648.86 Da
• Isoelectric Point: 9.6927

>T142792 WP_020996118.1 NZ_CP045837:c4775095-4774721 [Citrobacter sp. H12-3-2]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEHGMKLPDAIILATAQIHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T142792 NZ_CP060868:c70248-69832 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9479.80 Da
• Isoelectric Point: 4.3437

>AT142792 WP_020996119.1 NZ_CP045837:c4775337-4775095 [Citrobacter sp. H12-3-2]
MMVGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT142792 NZ_CP060868:c70475-70245 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA