Detailed information of TA system TA142789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4212887..4213463
• Replicon: chromosome
• Accession: NZ_CP045837
• Organism: Citrobacter sp. H12-3-2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: GHC22_RS20060
• Protein ID: WP_153753781.1
• Coordinates: 4213176..4213463 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: GHC22_RS20055
• Protein ID: WP_153753780.1
• Coordinates: 4212887..4213189 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC22_RS20035	4208575..4209117	+	543	WP_003830032.1	hypothetical protein	-
GHC22_RS20040	4209407..4211557	+	2151	WP_003830033.1	pyruvate/proton symporter BtsT	-
GHC22_RS20045	4211608..4211802	+	195	WP_003830038.1	YbdD/YjiX family protein	-
GHC22_RS20050	4211815..4212771	+	957	WP_153753779.1	GTPase	-
GHC22_RS20055	4212887..4213189	-	303	WP_153753780.1	BrnA antitoxin family protein	Antitoxin
GHC22_RS20060	4213176..4213463	-	288	WP_153753781.1	BrnT family toxin	Toxin
GHC22_RS20065	4213817..4216162	+	2346	WP_153753782.1	type I restriction-modification system subunit M	-
GHC22_RS20070	4216162..4217472	+	1311	WP_153753783.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11193.64 Da
• Isoelectric Point: 8.1209

>T142789 WP_153753781.1 NZ_CP045837:c4213463-4213176 [Citrobacter sp. H12-3-2]
MPMEFEWGANKAQSNHRKHGVRFEDAVLVFDDPRHMSRQDRYENGEYRWQTIGIVHCIVVILVAHSVRFESGVEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T142789 NZ_CP060867:c4019027-4018713 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11516.07 Da
• Isoelectric Point: 9.5646

>AT142789 WP_153753780.1 NZ_CP045837:c4213189-4212887 [Citrobacter sp. H12-3-2]
MSMVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRSKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT142789 NZ_CP060867:c4018716-4018300 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA