Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3662018..3662638 Replicon chromosome
Accession NZ_CP045837
Organism Citrobacter sp. H12-3-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GHC22_RS17545 Protein ID WP_002892050.1
Coordinates 3662420..3662638 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag GHC22_RS17540 Protein ID WP_003021733.1
Coordinates 3662018..3662392 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHC22_RS17530 3657164..3658357 + 1194 WP_020996573.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GHC22_RS17535 3658380..3661529 + 3150 WP_008783964.1 multidrug efflux RND transporter permease subunit -
GHC22_RS17540 3662018..3662392 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
GHC22_RS17545 3662420..3662638 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GHC22_RS17550 3662820..3663371 + 552 WP_003835924.1 maltose O-acetyltransferase -
GHC22_RS17555 3663488..3663958 + 471 WP_020996574.1 YlaC family protein -
GHC22_RS17560 3664036..3664176 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
GHC22_RS17565 3664178..3664438 - 261 WP_153753648.1 type B 50S ribosomal protein L31 -
GHC22_RS17570 3664627..3666180 + 1554 WP_153753649.1 EAL domain-containing protein -
GHC22_RS17575 3666232..3666585 - 354 WP_008783959.1 DUF1428 family protein -
GHC22_RS17580 3666650..3667279 - 630 WP_032942547.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T142788 WP_002892050.1 NZ_CP045837:3662420-3662638 [Citrobacter sp. H12-3-2]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T142788 NZ_CP060867:c3953386-3953090 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT142788 WP_003021733.1 NZ_CP045837:3662018-3662392 [Citrobacter sp. H12-3-2]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT142788 NZ_CP060867:c3953088-3952693 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References