Detailed information of TA system TA142776

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 867979..868633
• Replicon: chromosome
• Accession: NZ_CP045837
• Organism: Citrobacter sp. H12-3-2

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: GHC22_RS04185
• Protein ID: WP_003825515.1
• Coordinates: 868226..868633 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: GHC22_RS04180
• Protein ID: WP_003825514.1
• Coordinates: 867979..868245 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC22_RS04155	863134..864567	-	1434	WP_103284743.1	6-phospho-beta-glucosidase BglA	-
GHC22_RS04160	864688..865416	-	729	WP_153753956.1	MurR/RpiR family transcriptional regulator	-
GHC22_RS04165	865469..865780	+	312	WP_048225466.1	N(4)-acetylcytidine aminohydrolase	-
GHC22_RS04170	865944..866606	+	663	WP_071445549.1	hemolysin III family protein	-
GHC22_RS04175	866738..867721	-	984	WP_153752296.1	tRNA-modifying protein YgfZ	-
GHC22_RS04180	867979..868245	+	267	WP_003825514.1	FAD assembly factor SdhE	Antitoxin
GHC22_RS04185	868226..868633	+	408	WP_003825515.1	protein YgfX	Toxin
GHC22_RS04190	868735..869256	-	522	WP_003026933.1	flavodoxin FldB	-
GHC22_RS04195	869370..870266	+	897	WP_003825519.1	site-specific tyrosine recombinase XerD	-
GHC22_RS04200	870290..871003	+	714	WP_003825520.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GHC22_RS04205	871009..872742	+	1734	WP_153752298.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15882.76 Da
• Isoelectric Point: 11.3523

>T142776 WP_003825515.1 NZ_CP045837:868226-868633 [Citrobacter sp. H12-3-2]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQG

Nucleotide

• Length: 408 bp

>T142776 NZ_CP060867:c1992440-1992333 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10615.14 Da
• Isoelectric Point: 5.1700

>AT142776 WP_003825514.1 NZ_CP045837:867979-868245 [Citrobacter sp. H12-3-2]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADEQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT142776 NZ_CP060867:1992488-1992554 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC