Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 686637..687316 Replicon chromosome
Accession NZ_CP045837
Organism Citrobacter sp. H12-3-2

Toxin (Protein)


Gene name ykfI Uniprot ID -
Locus tag GHC22_RS03320 Protein ID WP_106929604.1
Coordinates 686975..687316 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID -
Locus tag GHC22_RS03315 Protein ID WP_106929602.1
Coordinates 686637..686954 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHC22_RS03270 682069..682890 + 822 WP_153752214.1 DUF945 domain-containing protein -
GHC22_RS03275 683107..683808 + 702 WP_153752216.1 WYL domain-containing protein -
GHC22_RS03280 683849..684085 + 237 WP_032181217.1 hypothetical protein -
GHC22_RS03285 684085..684528 + 444 WP_153752218.1 hypothetical protein -
GHC22_RS03290 684552..685019 + 468 WP_106929591.1 hypothetical protein -
GHC22_RS03295 685096..685335 + 240 WP_106929593.1 DUF905 domain-containing protein -
GHC22_RS03300 685437..685895 + 459 WP_106929595.1 antirestriction protein -
GHC22_RS03305 685911..686387 + 477 WP_106929597.1 RadC family protein -
GHC22_RS03310 686396..686617 + 222 WP_106929599.1 DUF987 domain-containing protein -
GHC22_RS03315 686637..686954 + 318 WP_106929602.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GHC22_RS03320 686975..687316 + 342 WP_106929604.1 TA system toxin CbtA family protein Toxin
GHC22_RS03330 687679..688185 + 507 WP_008785849.1 G/U mismatch-specific DNA glycosylase -
GHC22_RS03335 688231..690078 - 1848 WP_003024699.1 RNA polymerase sigma factor RpoD -
GHC22_RS03340 690363..692108 - 1746 WP_003828467.1 DNA primase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 660392..687316 26924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12925.98 Da        Isoelectric Point: 9.1225

>T142775 WP_106929604.1 NZ_CP045837:686975-687316 [Citrobacter sp. H12-3-2]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLQQCRKNSVR

Download         Length: 342 bp

>T142775 NZ_CP060867:c1181131-1180913 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12039.63 Da        Isoelectric Point: 7.0917

>AT142775 WP_106929602.1 NZ_CP045837:686637-686954 [Citrobacter sp. H12-3-2]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGRFSDAECRKLDETFPHFIRQMESMLATGEVSPQHAHCVTLYH
NDFTCEADTLGSCGYVYIAVYHTQR

Download         Length: 318 bp

>AT142775 NZ_CP060867:c1181531-1181157 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References