Detailed information of TA system TA142772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 24528..26179
• Replicon: plasmid pAUSMDU00010541_01
• Accession: NZ_CP045833
• Organism: Neisseria gonorrhoeae strain AUSMDU00010541

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: D5K9G7
• Locus tag: GIJ09_RS11965
• Protein ID: WP_003701905.1
• Coordinates: 24528..25733 (+)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: GIJ09_RS11970
• Protein ID: WP_003701903.1
• Coordinates: 25748..26179 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIJ09_RS11940	21441..21557	-	117	WP_001791010.1	tetracycline resistance determinant leader peptide	-
GIJ09_RS11945	21945..22466	+	522	WP_003701911.1	hypothetical protein	-
GIJ09_RS11950	22849..23097	+	249	WP_029671738.1	hypothetical protein	-
GIJ09_RS11955	23094..24302	+	1209	WP_003701908.1	zeta toxin family protein	-
GIJ09_RS11960	24336..24521	+	186	WP_003701907.1	hypothetical protein	-
GIJ09_RS11965	24528..25733	+	1206	WP_003701905.1	toxin	Toxin
GIJ09_RS11970	25748..26179	+	432	WP_003701903.1	hypothetical protein	Antitoxin
GIJ09_RS11975	26231..26785	+	555	WP_003701901.1	recombinase family protein	-
GIJ09_RS11980	26895..27185	-	291	WP_003701899.1	virulence factor	-
GIJ09_RS11985	27288..27887	-	600	WP_003701898.1	TrbN protein	-
GIJ09_RS11990	27887..28405	-	519	WP_025456271.1	TrbM protein	-
GIJ09_RS11995	28452..30080	-	1629	WP_003701894.1	P-type conjugative transfer protein TrbL	-
GIJ09_RS12000	30294..31070	-	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M)	-	1..41998	41998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44675.53 Da
• Isoelectric Point: 7.7756

>T142772 WP_003701905.1 NZ_CP045833:24528-25733 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGRGASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T142772 NZ_CP060867:546604-546891 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT142772 WP_003701903.1 NZ_CP045833:25748-26179 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT142772 NZ_CP060867:546878-547180 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 6EPI
• PDB: 6EPG
• PDB: 6EPH

Antitoxin

• AlphaFold DB: D5K9G8