Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5033929..5034426 Replicon chromosome
Accession NZ_CP045827
Organism Escherichia coli strain AUSMDU00014361

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GIJ02_RS25385 Protein ID WP_001173343.1
Coordinates 5033929..5034102 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GIJ02_RS25390 Protein ID WP_000398619.1
Coordinates 5034130..5034426 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIJ02_RS25375 5030599..5032056 + 1458 WP_153781761.1 dipeptide/tripeptide permease DtpC -
GIJ02_RS25380 5032293..5033810 + 1518 WP_001295074.1 lysine--tRNA ligase -
GIJ02_RS25385 5033929..5034102 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GIJ02_RS25390 5034130..5034426 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GIJ02_RS25395 5034653..5034925 - 273 WP_000405647.1 N-acetyltransferase -
GIJ02_RS25400 5034937..5035167 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
GIJ02_RS25405 5035348..5036979 + 1632 WP_001216487.1 sensor histidine kinase -
GIJ02_RS25410 5036976..5037695 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espL2 / nleB1 / nleE / efa1 / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae / cesT / tir / map / cesF / espH / sepQ/escQ / escO / escN / escV / cesL / sepZ/espZ / escI / escJ / sepD / escC / cesD / etgA / escU / escT / escS / escR / escL / escK / cesAB / escE / espG 4967591..5034426 66835


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T142767 WP_001173343.1 NZ_CP045827:c5034102-5033929 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T142767 NZ_CP060863:47853-48203 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT142767 WP_000398619.1 NZ_CP045827:c5034426-5034130 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT142767 NZ_CP060863:47596-47859 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References