142764

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4169982..4170819 Replicon chromosome
Accession NZ_CP045827
Organism Escherichia coli strain AUSMDU00014361

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag GIJ02_RS21295 Protein ID WP_000227784.1
Coordinates 4170277..4170819 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GIJ02_RS21290 Protein ID WP_001297137.1
Coordinates 4169982..4170293 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIJ02_RS21265 4165002..4165949 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
GIJ02_RS21270 4165971..4167962 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GIJ02_RS21275 4167952..4168566 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GIJ02_RS21280 4168566..4168895 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GIJ02_RS21285 4168907..4169797 + 891 WP_000971336.1 heme o synthase -
GIJ02_RS21290 4169982..4170293 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GIJ02_RS21295 4170277..4170819 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
GIJ02_RS21300 4170875..4171810 - 936 WP_001375746.1 sel1 repeat family protein -
GIJ02_RS21305 4172218..4173582 + 1365 WP_001000977.1 MFS transporter -
GIJ02_RS21310 4173710..4174201 - 492 WP_001138904.1 nucleotide binding protein YajQ -
GIJ02_RS21315 4174369..4175280 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T142764 WP_000227784.1 NZ_CP045827:4170277-4170819 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T142764 NZ_CP060861:200407-200673 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT142764 WP_001297137.1 NZ_CP045827:4169982-4170293 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT142764 NZ_CP060861:200144-200407 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References