Detailed information of TA system TA142761

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3416871..3417706
• Replicon: chromosome
• Accession: NZ_CP045827
• Organism: Escherichia coli strain AUSMDU00014361

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GIJ02_RS17505
• Protein ID: WP_153781683.1
• Coordinates: 3417329..3417706 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: GIJ02_RS17500
• Protein ID: WP_001295723.1
• Coordinates: 3416871..3417239 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIJ02_RS17460	3412978..3413325	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
GIJ02_RS17465	3413322..3413726	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
GIJ02_RS17470	3413815..3414156	+	342	WP_072097794.1	hypothetical protein	-
GIJ02_RS17475	3414235..3414468	+	234	WP_001119729.1	DUF905 family protein	-
GIJ02_RS17480	3414568..3415386	+	819	WP_001234620.1	DUF945 domain-containing protein	-
GIJ02_RS17485	3415441..3415926	+	486	WP_000849582.1	antirestriction protein	-
GIJ02_RS17490	3415942..3416418	+	477	WP_001186725.1	RadC family protein	-
GIJ02_RS17495	3416487..3416708	+	222	WP_000692323.1	DUF987 domain-containing protein	-
GIJ02_RS17500	3416871..3417239	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GIJ02_RS17505	3417329..3417706	+	378	WP_153781683.1	TA system toxin CbtA family protein	Toxin
GIJ02_RS17510	3417703..3417906	+	204	WP_000761705.1	hypothetical protein	-
GIJ02_RS17515	3417919..3418032	+	114	WP_001161660.1	DUF957 domain-containing protein	-
GIJ02_RS17520	3418191..3419219	+	1029	Protein_3442	IS110 family transposase	-
GIJ02_RS17530	3419673..3419855	+	183	WP_024167529.1	ethanolamine utilization protein	-
GIJ02_RS17535	3419956..3420285	-	330	WP_000450409.1	DUF496 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14163.22 Da
• Isoelectric Point: 7.3249

>T142761 WP_153781683.1 NZ_CP045827:3417329-3417706 [Escherichia coli]
MKTLPDTHVREVSCCPPLVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T142761 NZ_CP060855:c4488279-4487968 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT142761 WP_001295723.1 NZ_CP045827:3416871-3417239 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT142761 NZ_CP060855:c4487967-4487677 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGGTATTGA

Structures

Antitoxin

• AlphaFold DB: S1NM52