Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2714581..2715219 Replicon chromosome
Accession NZ_CP045827
Organism Escherichia coli strain AUSMDU00014361

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GIJ02_RS13975 Protein ID WP_000813794.1
Coordinates 2714581..2714757 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GIJ02_RS13980 Protein ID WP_001270286.1
Coordinates 2714803..2715219 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIJ02_RS13955 2710200..2711414 - 1215 WP_001307190.1 BenE family transporter YdcO -
GIJ02_RS13960 2711467..2712003 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GIJ02_RS13965 2712076..2714037 + 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GIJ02_RS13970 2714129..2714359 - 231 WP_000494244.1 YncJ family protein -
GIJ02_RS13975 2714581..2714757 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GIJ02_RS13980 2714803..2715219 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GIJ02_RS13985 2715298..2716702 + 1405 Protein_2754 PLP-dependent aminotransferase family protein -
GIJ02_RS13990 2716947..2718092 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
GIJ02_RS13995 2718110..2719123 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GIJ02_RS14000 2719124..2720065 + 942 WP_153781634.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2709012..2710160 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T142752 WP_000813794.1 NZ_CP045827:2714581-2714757 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T142752 NZ_CP060852:4522249-4522560 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGATAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT142752 WP_001270286.1 NZ_CP045827:2714803-2715219 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT142752 NZ_CP060852:4522557-4523003 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References