142737

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1428409..1429325 Replicon chromosome
Accession NZ_CP045826
Organism Bacillus subtilis strain 73

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag GII76_RS07500 Protein ID WP_003244695.1
Coordinates 1428579..1429325 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag GII76_RS07495 Protein ID WP_003232646.1
Coordinates 1428409..1428579 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII76_RS07460 1425275..1425601 + 327 WP_029727046.1 XkdW family protein -
GII76_RS07465 1425598..1425762 + 165 WP_014479563.1 XkdX family protein -
GII76_RS07470 1425806..1426645 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
GII76_RS07475 1426698..1426967 + 270 WP_015252265.1 hemolysin XhlA family protein -
GII76_RS07480 1426980..1427243 + 264 WP_014479566.1 phage holin -
GII76_RS07485 1427256..1428149 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
GII76_RS07490 1428186..1428323 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
GII76_RS07495 1428409..1428579 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
GII76_RS07500 1428579..1429325 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
GII76_RS07505 1429435..1430436 - 1002 WP_003232642.1 inorganic phosphate transporter -
GII76_RS07510 1430449..1431066 - 618 WP_003218470.1 DUF47 domain-containing protein -
GII76_RS07515 1431342..1432658 - 1317 WP_015252262.1 serine/threonine exchanger -
GII76_RS07520 1433046..1433996 + 951 WP_003232637.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T142737 WP_003244695.1 NZ_CP045826:c1429325-1428579 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T142737 NZ_CP060849:c1393312-1393001 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGATAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT142737 WP_003232646.1 NZ_CP045826:c1428579-1428409 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT142737 NZ_CP060849:c1393004-1392558 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References