Detailed information of TA system TA142736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 573723..574359
• Replicon: chromosome
• Accession: NZ_CP045826
• Organism: Bacillus subtilis strain 73

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GII76_RS02985
• Protein ID: WP_003156187.1
• Coordinates: 574009..574359 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: GII76_RS02980
• Protein ID: WP_003225183.1
• Coordinates: 573723..574004 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII76_RS02960	570083..570682	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
GII76_RS02965	570777..571142	+	366	WP_015252768.1	holo-ACP synthase	-
GII76_RS02970	571308..572324	+	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
GII76_RS02975	572438..573607	+	1170	WP_029727188.1	alanine racemase	-
GII76_RS02980	573723..574004	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GII76_RS02985	574009..574359	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GII76_RS02990	574474..575298	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
GII76_RS02995	575303..575668	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
GII76_RS03000	575672..576073	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
GII76_RS03005	576085..577092	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
GII76_RS03010	577154..577483	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
GII76_RS03015	577480..577962	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
GII76_RS03020	577928..578716	+	789	WP_029727187.1	RNA polymerase sigma factor SigB	-
GII76_RS03025	578716..579315	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T142736 WP_003156187.1 NZ_CP045826:574009-574359 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T142736 NZ_CP060849:c986669-986184 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT142736 WP_003225183.1 NZ_CP045826:573723-574004 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT142736 NZ_CP060849:c986944-986657 [Salmonella enterica]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATTAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32