Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 573723..574359 Replicon chromosome
Accession NZ_CP045826
Organism Bacillus subtilis strain 73

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GII76_RS02985 Protein ID WP_003156187.1
Coordinates 574009..574359 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GII76_RS02980 Protein ID WP_003225183.1
Coordinates 573723..574004 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII76_RS02960 570083..570682 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GII76_RS02965 570777..571142 + 366 WP_015252768.1 holo-ACP synthase -
GII76_RS02970 571308..572324 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
GII76_RS02975 572438..573607 + 1170 WP_029727188.1 alanine racemase -
GII76_RS02980 573723..574004 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GII76_RS02985 574009..574359 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GII76_RS02990 574474..575298 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GII76_RS02995 575303..575668 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GII76_RS03000 575672..576073 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GII76_RS03005 576085..577092 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GII76_RS03010 577154..577483 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GII76_RS03015 577480..577962 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GII76_RS03020 577928..578716 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
GII76_RS03025 578716..579315 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142736 WP_003156187.1 NZ_CP045826:574009-574359 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142736 NZ_CP060849:c986669-986184 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT142736 WP_003225183.1 NZ_CP045826:573723-574004 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142736 NZ_CP060849:c986944-986657 [Salmonella enterica]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATTAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References