Detailed information of TA system TA142735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1434366..1435282
• Replicon: chromosome
• Accession: NZ_CP045825
• Organism: Bacillus subtilis strain 75

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: GII77_RS07680
• Protein ID: WP_015252263.1
• Coordinates: 1434536..1435282 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII77_RS07675
• Protein ID: WP_003232646.1
• Coordinates: 1434366..1434536 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII77_RS07640	1431226..1431555	+	330	WP_015252268.1	XkdW family protein	-
GII77_RS07645	1431552..1431716	+	165	WP_015252267.1	XkdX family protein	-
GII77_RS07650	1431763..1432602	+	840	WP_015252266.1	phage-like element PBSX protein XepA	-
GII77_RS07655	1432655..1432924	+	270	WP_015252265.1	hemolysin XhlA family protein	-
GII77_RS07660	1432937..1433200	+	264	WP_003232653.1	phage holin	-
GII77_RS07665	1433213..1434106	+	894	WP_014479567.1	N-acetylmuramoyl-L-alanine amidase	-
GII77_RS07670	1434143..1434280	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII77_RS07675	1434366..1434536	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII77_RS07680	1434536..1435282	-	747	WP_015252263.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII77_RS07685	1435392..1436393	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII77_RS07690	1436406..1437023	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII77_RS07695	1437299..1438615	-	1317	WP_015252262.1	serine/threonine exchanger	-
GII77_RS07700	1439004..1439954	+	951	WP_015252261.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29060.56 Da
• Isoelectric Point: 4.7741

>T142735 WP_015252263.1 NZ_CP045825:c1435282-1434536 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNKMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142735 NZ_CP060849:c882742-882389 [Salmonella enterica]
ATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGGCATGCCTGATCCTGG
GCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGTGGCTGCTGGCGGCAA
TGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTTATTACGCTGCTGTTT
TTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGCGGCAGGGCGGCTTAC
GCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142735 WP_003232646.1 NZ_CP045825:c1434536-1434366 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142735 NZ_CP060849:c882976-882755 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: G4EXD8