Detailed information of TA system TA142733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 523361..523997
• Replicon: chromosome
• Accession: NZ_CP045825
• Organism: Bacillus subtilis strain 75

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GII77_RS02680
• Protein ID: WP_003156187.1
• Coordinates: 523647..523997 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: GII77_RS02675
• Protein ID: WP_003225183.1
• Coordinates: 523361..523642 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII77_RS02655	519720..520319	-	600	WP_015252769.1	rhomboid family intramembrane serine protease	-
GII77_RS02660	520414..520779	+	366	WP_015252768.1	holo-ACP synthase	-
GII77_RS02665	520945..521961	+	1017	WP_080019556.1	outer membrane lipoprotein carrier protein LolA	-
GII77_RS02670	522076..523245	+	1170	WP_015252766.1	alanine racemase	-
GII77_RS02675	523361..523642	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GII77_RS02680	523647..523997	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GII77_RS02685	524112..524936	+	825	WP_015252765.1	RsbT co-antagonist protein RsbRA	-
GII77_RS02690	524941..525306	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
GII77_RS02695	525310..525711	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
GII77_RS02700	525723..526730	+	1008	WP_015252763.1	phosphoserine phosphatase RsbU	-
GII77_RS02705	526792..527121	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
GII77_RS02710	527118..527600	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
GII77_RS02715	527566..528354	+	789	WP_044052630.1	RNA polymerase sigma factor SigB	-
GII77_RS02720	528354..528953	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T142733 WP_003156187.1 NZ_CP045825:523647-523997 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T142733 NZ_CP060849:161014-161541 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGGGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTACTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT142733 WP_003225183.1 NZ_CP045825:523361-523642 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT142733 NZ_CP060849:160727-161017 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32