Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1329945..1330861 Replicon chromosome
Accession NZ_CP045824
Organism Bacillus subtilis strain MB8_B10

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag GII78_RS06945 Protein ID WP_003244695.1
Coordinates 1330115..1330861 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag GII78_RS06940 Protein ID WP_003232646.1
Coordinates 1329945..1330115 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII78_RS06905 1326811..1327137 + 327 WP_069703538.1 XkdW family protein -
GII78_RS06910 1327134..1327298 + 165 WP_014479563.1 XkdX family protein -
GII78_RS06915 1327342..1328181 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
GII78_RS06920 1328234..1328503 + 270 WP_015252265.1 hemolysin XhlA family protein -
GII78_RS06925 1328516..1328779 + 264 WP_014479566.1 phage holin -
GII78_RS06930 1328792..1329685 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
GII78_RS06935 1329722..1329859 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
GII78_RS06940 1329945..1330115 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
GII78_RS06945 1330115..1330861 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
GII78_RS06950 1330971..1331972 - 1002 WP_003232642.1 inorganic phosphate transporter -
GII78_RS06955 1331985..1332602 - 618 WP_003218470.1 DUF47 domain-containing protein -
GII78_RS06960 1332878..1334194 - 1317 WP_029317680.1 serine/threonine exchanger -
GII78_RS06965 1334583..1335533 + 951 WP_014476561.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T142722 WP_003244695.1 NZ_CP045824:c1330861-1330115 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T142722 NZ_CP060844:c3584692-3584589 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT142722 WP_003232646.1 NZ_CP045824:c1330115-1329945 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT142722 NZ_CP060844:3584587-3584730 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References