Detailed information of TA system TA142722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1329945..1330861
• Replicon: chromosome
• Accession: NZ_CP045824
• Organism: Bacillus subtilis strain MB8_B10

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII78_RS06945
• Protein ID: WP_003244695.1
• Coordinates: 1330115..1330861 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII78_RS06940
• Protein ID: WP_003232646.1
• Coordinates: 1329945..1330115 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII78_RS06905	1326811..1327137	+	327	WP_069703538.1	XkdW family protein	-
GII78_RS06910	1327134..1327298	+	165	WP_014479563.1	XkdX family protein	-
GII78_RS06915	1327342..1328181	+	840	WP_029727045.1	phage-like element PBSX protein XepA	-
GII78_RS06920	1328234..1328503	+	270	WP_015252265.1	hemolysin XhlA family protein	-
GII78_RS06925	1328516..1328779	+	264	WP_014479566.1	phage holin	-
GII78_RS06930	1328792..1329685	+	894	WP_029727044.1	N-acetylmuramoyl-L-alanine amidase	-
GII78_RS06935	1329722..1329859	-	138	WP_119899277.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII78_RS06940	1329945..1330115	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII78_RS06945	1330115..1330861	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII78_RS06950	1330971..1331972	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII78_RS06955	1331985..1332602	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII78_RS06960	1332878..1334194	-	1317	WP_029317680.1	serine/threonine exchanger	-
GII78_RS06965	1334583..1335533	+	951	WP_014476561.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T142722 WP_003244695.1 NZ_CP045824:c1330861-1330115 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142722 NZ_CP060844:c3584692-3584589 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142722 WP_003232646.1 NZ_CP045824:c1330115-1329945 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142722 NZ_CP060844:3584587-3584730 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• AlphaFold DB: G4EXD8