Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516632..517268 Replicon chromosome
Accession NZ_CP045823
Organism Bacillus subtilis strain MB8_B1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GII79_RS02625 Protein ID WP_003156187.1
Coordinates 516918..517268 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GII79_RS02620 Protein ID WP_003225183.1
Coordinates 516632..516913 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII79_RS02600 512992..513591 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GII79_RS02605 513686..514051 + 366 WP_015252768.1 holo-ACP synthase -
GII79_RS02610 514217..515233 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
GII79_RS02615 515347..516516 + 1170 WP_029727188.1 alanine racemase -
GII79_RS02620 516632..516913 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GII79_RS02625 516918..517268 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GII79_RS02630 517383..518207 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GII79_RS02635 518212..518577 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GII79_RS02640 518581..518982 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GII79_RS02645 518994..520001 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GII79_RS02650 520063..520392 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GII79_RS02655 520389..520871 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GII79_RS02660 520837..521625 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
GII79_RS02665 521625..522224 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142705 WP_003156187.1 NZ_CP045823:516918-517268 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142705 NZ_CP060840:845390-845803 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT142705 WP_003225183.1 NZ_CP045823:516632-516913 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142705 NZ_CP060840:845143-845409 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References