Detailed information of TA system TA142705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 516632..517268
• Replicon: chromosome
• Accession: NZ_CP045823
• Organism: Bacillus subtilis strain MB8_B1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GII79_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 516918..517268 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: GII79_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 516632..516913 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII79_RS02600	512992..513591	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
GII79_RS02605	513686..514051	+	366	WP_015252768.1	holo-ACP synthase	-
GII79_RS02610	514217..515233	+	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
GII79_RS02615	515347..516516	+	1170	WP_029727188.1	alanine racemase	-
GII79_RS02620	516632..516913	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GII79_RS02625	516918..517268	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GII79_RS02630	517383..518207	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
GII79_RS02635	518212..518577	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
GII79_RS02640	518581..518982	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
GII79_RS02645	518994..520001	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
GII79_RS02650	520063..520392	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
GII79_RS02655	520389..520871	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
GII79_RS02660	520837..521625	+	789	WP_029727187.1	RNA polymerase sigma factor SigB	-
GII79_RS02665	521625..522224	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T142705 WP_003156187.1 NZ_CP045823:516918-517268 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T142705 NZ_CP060840:845390-845803 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT142705 WP_003225183.1 NZ_CP045823:516632-516913 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT142705 NZ_CP060840:845143-845409 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32