Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518254..518890 Replicon chromosome
Accession NZ_CP045821
Organism Bacillus subtilis strain MB8_B7

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GII80_RS02640 Protein ID WP_003156187.1
Coordinates 518540..518890 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GII80_RS02635 Protein ID WP_003225183.1
Coordinates 518254..518535 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII80_RS02615 514613..515212 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GII80_RS02620 515307..515672 + 366 WP_003234281.1 holo-ACP synthase -
GII80_RS02625 515838..516854 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
GII80_RS02630 516969..518138 + 1170 WP_003234284.1 alanine racemase -
GII80_RS02635 518254..518535 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GII80_RS02640 518540..518890 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GII80_RS02645 519005..519829 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GII80_RS02650 519834..520199 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GII80_RS02655 520203..520604 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GII80_RS02660 520616..521623 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GII80_RS02665 521685..522014 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GII80_RS02670 522011..522493 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GII80_RS02675 522459..523247 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
GII80_RS02680 523247..523846 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142682 WP_003156187.1 NZ_CP045821:518540-518890 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142682 NZ_CP060825:3213130-3213390 [Streptomyces sp. CRPJ-33]
GTGAAGTTCGTGTGGGACGAGTCGAGCTGGGGCGACTACGTCTGGTGGCAGGCCCAGGACCGCAAGGTCCTGAAGCGCAT
CAACACCCTGCTCCAGGACATCGCACGCAACGGCAACGAAGGCATCGGGAAGCCCGAGCCCCTCAAGCACGGCTTCCAGG
GCTACTGGTCGCGCCGCATCACCGACGAGCACCGCCTCGTCTACAAGATCGTCGACGACGAGATCCGGGTCGCCGCCTGC
CGGTACCACTACGAGCGCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT142682 WP_003225183.1 NZ_CP045821:518254-518535 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142682 NZ_CP060825:3212882-3213133 [Streptomyces sp. CRPJ-33]
ATGCGGACCATGACCTACACCGAGTCCCGCGCGAAGTACGCCGAGACCCTGAACGCGGTGGTCGACGATCGCGAGGAGGT
CATAGTGACCAGAGCGGGGCGCGAGCCCGTGGTCATCGTCGCGCTCGCCGAGTACGAGTCGTTGAAGGAGACCGCGTACC
TGCTGCGCAGCCCGGAGAACGCCCGGCGCCTGCTGGCATCCATCGACCGCCTGGAGAACGGCGGCGGCACGGTCAGGGAG
CTCGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References