Detailed information of TA system TA142667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1319552..1320468
• Replicon: chromosome
• Accession: NZ_CP045820
• Organism: Bacillus subtilis strain MB9_B1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII81_RS06905
• Protein ID: WP_003244695.1
• Coordinates: 1319722..1320468 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII81_RS06900
• Protein ID: WP_003232646.1
• Coordinates: 1319552..1319722 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII81_RS06865	1316418..1316744	+	327	WP_029727046.1	XkdW family protein	-
GII81_RS06870	1316741..1316905	+	165	WP_014479563.1	XkdX family protein	-
GII81_RS06875	1316949..1317788	+	840	WP_029727045.1	phage-like element PBSX protein XepA	-
GII81_RS06880	1317841..1318110	+	270	WP_015252265.1	hemolysin XhlA family protein	-
GII81_RS06885	1318123..1318386	+	264	WP_014479566.1	phage holin	-
GII81_RS06890	1318399..1319292	+	894	WP_029727044.1	N-acetylmuramoyl-L-alanine amidase	-
GII81_RS06895	1319329..1319466	-	138	WP_119899277.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII81_RS06900	1319552..1319722	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII81_RS06905	1319722..1320468	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII81_RS06910	1320578..1321579	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII81_RS06915	1321592..1322209	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII81_RS06920	1322485..1323801	-	1317	WP_015252262.1	serine/threonine exchanger	-
GII81_RS06925	1324189..1325139	+	951	WP_003232637.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1284499..1384954	100455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T142667 WP_003244695.1 NZ_CP045820:c1320468-1319722 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142667 NZ_CP060815:c11242-10955 [Acinetobacter variabilis]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTTGAAACTGCCAG
CCTTGTATTTGAAGATCCTCTGAGGATCTCAATCCAAGACAGACATACCAATGGGGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGGCTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142667 WP_003232646.1 NZ_CP045820:c1319722-1319552 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142667 NZ_CP060815:c10968-10654 [Acinetobacter variabilis]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGTC
ATAAACAGTTCTATCGTCCTGTGAAGCAACAAACTTCCATTCGTTTAGACGCGGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTTTACGAGATGCTATGCTCAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8