142666

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516643..517279 Replicon chromosome
Accession NZ_CP045820
Organism Bacillus subtilis strain MB9_B1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GII81_RS02625 Protein ID WP_003156187.1
Coordinates 516929..517279 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GII81_RS02620 Protein ID WP_003225183.1
Coordinates 516643..516924 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII81_RS02600 513003..513602 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GII81_RS02605 513697..514062 + 366 WP_015252768.1 holo-ACP synthase -
GII81_RS02610 514228..515244 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
GII81_RS02615 515358..516527 + 1170 WP_029727188.1 alanine racemase -
GII81_RS02620 516643..516924 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GII81_RS02625 516929..517279 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GII81_RS02630 517394..518218 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GII81_RS02635 518223..518588 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GII81_RS02640 518592..518993 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GII81_RS02645 519005..520012 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GII81_RS02650 520074..520403 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GII81_RS02655 520400..520882 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GII81_RS02660 520848..521636 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
GII81_RS02665 521636..522235 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142666 WP_003156187.1 NZ_CP045820:516929-517279 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142666 NZ_CP060814:c15636-15349 [Acinetobacter variabilis]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTCTTTGATGATCCATTACGGATCTCAATCCAGGATAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
GAGTAAAAGGCGTACTAATGCTATTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAGGTAACTAAAGCAGAGCGAGATAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT142666 WP_003225183.1 NZ_CP045820:516643-516924 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142666 NZ_CP060814:c15362-15048 [Acinetobacter variabilis]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTTAGTGAGAAGCAAAATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
ATAATCAGTTCTACCGTCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAATCTCAA
GGCAAAGGTTATCAAACACGAATGAATGAAATCTTGCGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References