142665

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1338157..1339073 Replicon chromosome
Accession NZ_CP045819
Organism Bacillus subtilis strain MB9_B4

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag GII82_RS06975 Protein ID WP_003244695.1
Coordinates 1338327..1339073 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag GII82_RS06970 Protein ID WP_003232646.1
Coordinates 1338157..1338327 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII82_RS06935 1335023..1335349 + 327 WP_029727046.1 XkdW family protein -
GII82_RS06940 1335346..1335510 + 165 WP_014479563.1 XkdX family protein -
GII82_RS06945 1335554..1336393 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
GII82_RS06950 1336446..1336715 + 270 WP_015252265.1 hemolysin XhlA family protein -
GII82_RS06955 1336728..1336991 + 264 WP_014479566.1 phage holin -
GII82_RS06960 1337004..1337897 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
GII82_RS06965 1337934..1338071 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
GII82_RS06970 1338157..1338327 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
GII82_RS06975 1338327..1339073 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
GII82_RS06980 1339183..1340184 - 1002 WP_003232642.1 inorganic phosphate transporter -
GII82_RS06985 1340197..1340814 - 618 WP_003218470.1 DUF47 domain-containing protein -
GII82_RS06990 1341090..1342406 - 1317 WP_015252262.1 serine/threonine exchanger -
GII82_RS06995 1342794..1343744 + 951 WP_003232637.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T142665 WP_003244695.1 NZ_CP045819:c1339073-1338327 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T142665 NZ_CP060812:128523-128816 [Acinetobacter variabilis]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGAGATCAATTTAA
AAAGAAACTAGCTAAAGTCATAGAGCAGCCACATATTCCAAAGAACATGCTTAGAGGCGATCTTGCAGGTTGCTATAAAA
TCAAATTATTAAAAGCTGGTGTCCGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCCTGCTCATTACCGTCGGA
AAACGAGCTGATAGCATAGTTTATGACGAAGCGAAAAAGCGAATTAAAGACTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT142665 WP_003232646.1 NZ_CP045819:c1338327-1338157 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT142665 NZ_CP060812:128288-128533 [Acinetobacter variabilis]
ATGGCTACTGTTAACTTCAGGGTCGATGGAGCCCTTAAAGAAAAATCTTATTCAATTCTTAAAGAGCAAGGTATTGCACC
TACGGATTTCTTCACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTGCCCGTCAAAAAAGCTTTGCTTTCGGAAG
AAGATGAAGAATTATTAGCTCTTGTCCGTAAGCGCATTAACGATCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References