Detailed information of TA system TA142663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1319902..1320818
• Replicon: chromosome
• Accession: NZ_CP045818
• Organism: Bacillus subtilis strain MB9_B6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII83_RS06910
• Protein ID: WP_003244695.1
• Coordinates: 1320072..1320818 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII83_RS06905
• Protein ID: WP_003232646.1
• Coordinates: 1319902..1320072 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII83_RS06870	1316768..1317094	+	327	WP_029727046.1	XkdW family protein	-
GII83_RS06875	1317091..1317255	+	165	WP_014479563.1	XkdX family protein	-
GII83_RS06880	1317299..1318138	+	840	WP_029727045.1	phage-like element PBSX protein XepA	-
GII83_RS06885	1318191..1318460	+	270	WP_015252265.1	hemolysin XhlA family protein	-
GII83_RS06890	1318473..1318736	+	264	WP_014479566.1	phage holin	-
GII83_RS06895	1318749..1319642	+	894	WP_029727044.1	N-acetylmuramoyl-L-alanine amidase	-
GII83_RS06900	1319679..1319816	-	138	WP_119899277.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII83_RS06905	1319902..1320072	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII83_RS06910	1320072..1320818	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII83_RS06915	1320928..1321929	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII83_RS06920	1321942..1322559	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII83_RS06925	1322835..1324151	-	1317	WP_015252262.1	serine/threonine exchanger	-
GII83_RS06930	1324539..1325489	+	951	WP_003232637.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T142663 WP_003244695.1 NZ_CP045818:c1320818-1320072 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142663 NZ_CP060812:124771-125067 [Acinetobacter variabilis]
ATGATTGAAATTAAACGTCTGCCAGAATTTGATGAATGGTTAGATGGCATTAAAGACAATATGACCCGTATTCGTCTGAA
TCGTAGATTAGATAAGGTTCAACGTGGAAATTGGGGAGACATTAAGCCTCTCCAAGATGGCGTTTGGGAAATGAGAGAGT
TCTTCGGTGCTGGATGGAGAATGTATTACATCCAGCATGGTGACGTAGTGATTGTAATGCTTGGTGGAGGAGATAAATCG
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142663 WP_003232646.1 NZ_CP045818:c1320072-1319902 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142663 NZ_CP060812:125069-125359 [Acinetobacter variabilis]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TTGCTAAAGAAGCAGGGATTGGGCGAGAGGCACTCTACAAAGCCCTACGTCATGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCGTTAGGCTTAAAACTCACTGTACAACACGCCTAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8