Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516721..517357 Replicon chromosome
Accession NZ_CP045818
Organism Bacillus subtilis strain MB9_B6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GII83_RS02630 Protein ID WP_003156187.1
Coordinates 517007..517357 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GII83_RS02625 Protein ID WP_003225183.1
Coordinates 516721..517002 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII83_RS02605 513081..513680 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GII83_RS02610 513775..514140 + 366 WP_015252768.1 holo-ACP synthase -
GII83_RS02615 514306..515322 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
GII83_RS02620 515436..516605 + 1170 WP_029727188.1 alanine racemase -
GII83_RS02625 516721..517002 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GII83_RS02630 517007..517357 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GII83_RS02635 517472..518296 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GII83_RS02640 518301..518666 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GII83_RS02645 518670..519071 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GII83_RS02650 519083..520090 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GII83_RS02655 520152..520481 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GII83_RS02660 520478..520960 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GII83_RS02665 520926..521714 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
GII83_RS02670 521714..522313 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142662 WP_003156187.1 NZ_CP045818:517007-517357 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142662 NZ_CP060812:c123711-123391 [Acinetobacter variabilis]
ATGCTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTAAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAAGGTGACTTAATCAAGAATGGCGGTGGCATTCGTAAAGTACGCTGTGCTCAAGGTA
ATAAAGGTAAAAGTGGCGGTATCCGTGTGATCTATTACTGGGTCACAGAAGATGATCAGATATTTTTCTTGGTGGCTTAT
CCAAAGTCTACGAAAGATAACCTGACAGACAAAGAGACCTCTATTTTGCGTCAATTAGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT142662 WP_003225183.1 NZ_CP045818:516721-517002 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142662 NZ_CP060812:c123398-123126 [Acinetobacter variabilis]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAACGCAATGAAATGAAAGCAAG
CCGGGTAACTGAGCTTGAATTGCCGGATATTAAGGAAGTACGTGAGAAGACAGGCTTAACCCAAGCTGAATTTGCTGCAC
GTTTGCATATCAGTGCCAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCAACTGGCCCAGCAGCAACGCTGATC
CGCATTCTGGATGCTCATCCAACCTTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References