Detailed information of TA system TA142661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1314384..1315300
• Replicon: chromosome
• Accession: NZ_CP045817
• Organism: Bacillus subtilis strain P5_B1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII85_RS06860
• Protein ID: WP_003244695.1
• Coordinates: 1314554..1315300 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII85_RS06855
• Protein ID: WP_003232646.1
• Coordinates: 1314384..1314554 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII85_RS06820	1311247..1311576	+	330	WP_041850928.1	XkdW family protein	-
GII85_RS06825	1311573..1311737	+	165	WP_041850927.1	XkdX family protein	-
GII85_RS06830	1311781..1312620	+	840	WP_041850926.1	phage-like element PBSX protein XepA	-
GII85_RS06835	1312673..1312942	+	270	WP_003232655.1	hemolysin XhlA family protein	-
GII85_RS06840	1312955..1313218	+	264	WP_003232653.1	phage holin	-
GII85_RS06845	1313231..1314124	+	894	WP_041850925.1	N-acetylmuramoyl-L-alanine amidase	-
GII85_RS06850	1314161..1314298	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII85_RS06855	1314384..1314554	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII85_RS06860	1314554..1315300	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII85_RS06865	1315410..1316411	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII85_RS06870	1316424..1317041	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII85_RS06875	1317317..1318633	-	1317	WP_041850924.1	serine/threonine exchanger	-
GII85_RS06880	1319021..1319971	+	951	WP_041344800.1	ring-cleaving dioxygenase	-
GII85_RS06885	1320072..1320218	+	147	WP_120363335.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T142661 WP_003244695.1 NZ_CP045817:c1315300-1314554 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142661 NZ_CP060812:110359-110679 [Acinetobacter variabilis]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142661 WP_003232646.1 NZ_CP045817:c1314554-1314384 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142661 NZ_CP060812:110672-110944 [Acinetobacter variabilis]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8