Detailed information of TA system TA142655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1375797..1376713
• Replicon: chromosome
• Accession: NZ_CP045816
• Organism: Bacillus subtilis strain P5_B2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII86_RS07250
• Protein ID: WP_003244695.1
• Coordinates: 1375967..1376713 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII86_RS07245
• Protein ID: WP_003232646.1
• Coordinates: 1375797..1375967 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII86_RS07210	1372656..1372985	+	330	WP_046160312.1	XkdW family protein	-
GII86_RS07215	1372982..1373146	+	165	WP_014479563.1	XkdX family protein	-
GII86_RS07220	1373193..1374032	+	840	WP_014479564.1	phage-like element PBSX protein XepA	-
GII86_RS07225	1374085..1374354	+	270	WP_014479565.1	hemolysin XhlA family protein	-
GII86_RS07230	1374367..1374630	+	264	WP_014479566.1	phage holin	-
GII86_RS07235	1374643..1375536	+	894	WP_021479459.1	N-acetylmuramoyl-L-alanine amidase	-
GII86_RS07240	1375574..1375711	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII86_RS07245	1375797..1375967	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII86_RS07250	1375967..1376713	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII86_RS07255	1376823..1377824	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII86_RS07260	1377837..1378454	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII86_RS07265	1378730..1380046	-	1317	WP_014479570.1	serine/threonine exchanger	-
GII86_RS07270	1380435..1381385	+	951	WP_014479571.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T142655 WP_003244695.1 NZ_CP045816:c1376713-1375967 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142655 NZ_CP060808:c41874-41458 [Klebsiella variicola]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142655 WP_003232646.1 NZ_CP045816:c1375967-1375797 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142655 NZ_CP060808:c42101-41871 [Klebsiella variicola]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• AlphaFold DB: G4EXD8