Detailed information of TA system TA142654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 550108..550744
• Replicon: chromosome
• Accession: NZ_CP045816
• Organism: Bacillus subtilis strain P5_B2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GII86_RS02850
• Protein ID: WP_003156187.1
• Coordinates: 550394..550744 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: GII86_RS02845
• Protein ID: WP_003225183.1
• Coordinates: 550108..550389 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII86_RS02825	546467..547066	-	600	WP_041850724.1	rhomboid family intramembrane serine protease	-
GII86_RS02830	547161..547526	+	366	WP_015252768.1	holo-ACP synthase	-
GII86_RS02835	547692..548708	+	1017	WP_082090354.1	outer membrane lipoprotein carrier protein LolA	-
GII86_RS02840	548823..549992	+	1170	WP_015252766.1	alanine racemase	-
GII86_RS02845	550108..550389	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GII86_RS02850	550394..550744	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GII86_RS02855	550859..551683	+	825	WP_015252765.1	RsbT co-antagonist protein RsbRA	-
GII86_RS02860	551688..552053	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
GII86_RS02865	552057..552458	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
GII86_RS02870	552470..553477	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
GII86_RS02875	553539..553868	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
GII86_RS02880	553865..554347	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
GII86_RS02885	554313..555101	+	789	WP_029727187.1	RNA polymerase sigma factor SigB	-
GII86_RS02890	555101..555700	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T142654 WP_003156187.1 NZ_CP045816:550394-550744 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T142654 NZ_CP060807:c5380881-5380498 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT142654 WP_003225183.1 NZ_CP045816:550108-550389 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT142654 NZ_CP060807:c5381123-5380881 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32