Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 550108..550744 Replicon chromosome
Accession NZ_CP045816
Organism Bacillus subtilis strain P5_B2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GII86_RS02850 Protein ID WP_003156187.1
Coordinates 550394..550744 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GII86_RS02845 Protein ID WP_003225183.1
Coordinates 550108..550389 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII86_RS02825 546467..547066 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
GII86_RS02830 547161..547526 + 366 WP_015252768.1 holo-ACP synthase -
GII86_RS02835 547692..548708 + 1017 WP_082090354.1 outer membrane lipoprotein carrier protein LolA -
GII86_RS02840 548823..549992 + 1170 WP_015252766.1 alanine racemase -
GII86_RS02845 550108..550389 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GII86_RS02850 550394..550744 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GII86_RS02855 550859..551683 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
GII86_RS02860 551688..552053 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GII86_RS02865 552057..552458 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GII86_RS02870 552470..553477 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GII86_RS02875 553539..553868 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GII86_RS02880 553865..554347 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GII86_RS02885 554313..555101 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
GII86_RS02890 555101..555700 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142654 WP_003156187.1 NZ_CP045816:550394-550744 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142654 NZ_CP060807:c5380881-5380498 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT142654 WP_003225183.1 NZ_CP045816:550108-550389 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142654 NZ_CP060807:c5381123-5380881 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References