142630

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1348032..1348948 Replicon chromosome
Accession NZ_CP045812
Organism Bacillus subtilis strain P8_B3

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag GII89_RS07075 Protein ID WP_003244695.1
Coordinates 1348202..1348948 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag GII89_RS07070 Protein ID WP_003232646.1
Coordinates 1348032..1348202 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GII89_RS07035 1344895..1345224 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
GII89_RS07040 1345221..1345385 + 165 WP_003232658.1 XkdX family protein -
GII89_RS07045 1345429..1346268 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
GII89_RS07050 1346321..1346590 + 270 WP_003232655.1 hemolysin XhlA family protein -
GII89_RS07055 1346603..1346866 + 264 WP_003232653.1 phage holin -
GII89_RS07060 1346879..1347772 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
GII89_RS07065 1347809..1347946 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
GII89_RS07070 1348032..1348202 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
GII89_RS07075 1348202..1348948 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
GII89_RS07080 1349058..1350059 - 1002 WP_003232642.1 inorganic phosphate transporter -
GII89_RS07085 1350072..1350689 - 618 WP_003218470.1 DUF47 domain-containing protein -
GII89_RS07090 1350965..1352281 - 1317 WP_003244819.1 serine/threonine exchanger -
GII89_RS07095 1352670..1353620 + 951 WP_003245086.1 ring-cleaving dioxygenase -
GII89_RS07100 1353721..1353867 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T142630 WP_003244695.1 NZ_CP045812:c1348948-1348202 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T142630 NZ_CP060796:c2509507-2509406 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT142630 WP_003232646.1 NZ_CP045812:c1348202-1348032 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT142630 NZ_CP060796:2509283-2509461 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTTATTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCG
AAAATCAGTAACGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References