Detailed information of TA system TA142625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1327470..1328386
• Replicon: chromosome
• Accession: NZ_CP045811
• Organism: Bacillus subtilis strain P9_B1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII90_RS06945
• Protein ID: WP_003244695.1
• Coordinates: 1327640..1328386 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII90_RS06940
• Protein ID: WP_003232646.1
• Coordinates: 1327470..1327640 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII90_RS06905	1324333..1324662	+	330	WP_003232660.1	phage-like element PBSX protein XkdW	-
GII90_RS06910	1324659..1324823	+	165	WP_003232658.1	XkdX family protein	-
GII90_RS06915	1324867..1325706	+	840	WP_003245597.1	phage-like element PBSX protein XepA	-
GII90_RS06920	1325759..1326028	+	270	WP_003232655.1	hemolysin XhlA family protein	-
GII90_RS06925	1326041..1326304	+	264	WP_003232653.1	phage holin	-
GII90_RS06930	1326317..1327210	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
GII90_RS06935	1327247..1327384	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII90_RS06940	1327470..1327640	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII90_RS06945	1327640..1328386	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII90_RS06950	1328496..1329497	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII90_RS06955	1329510..1330127	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII90_RS06960	1330403..1331719	-	1317	WP_003244819.1	serine/threonine exchanger	-
GII90_RS06965	1332108..1333058	+	951	WP_003245086.1	ring-cleaving dioxygenase	-
GII90_RS06970	1333159..1333305	+	147	WP_003244977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T142625 WP_003244695.1 NZ_CP045811:c1328386-1327640 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T142625 NZ_CP060794:c2386378-2386016 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT142625 WP_003232646.1 NZ_CP045811:c1327640-1327470 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT142625 NZ_CP060794:c2386545-2386375 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• AlphaFold DB: G4EXD8