Detailed information of TA system TA142623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/MazF(toxin)
• Location: 4521343..4521911
• Replicon: chromosome
• Accession: NZ_CP045809
• Organism: Gordonia pseudamarae strain CON9

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: GII31_RS19730
• Protein ID: WP_260840128.1
• Coordinates: 4521343..4521690 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: -
• Locus tag: GII31_RS19735
• Protein ID: WP_213245050.1
• Coordinates: 4521678..4521911 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII31_RS19710 (GII31_19610)	4517068..4517664	-	597	WP_260840127.1	alpha/beta hydrolase	-
GII31_RS19715 (GII31_19615)	4517661..4518407	-	747	WP_213245046.1	nitroreductase	-
GII31_RS19720 (GII31_19620)	4518720..4520117	+	1398	WP_213245047.1	oxygenase MpaB family protein	-
GII31_RS19725 (GII31_19625)	4520114..4521274	+	1161	WP_213245048.1	CaiB/BaiF CoA-transferase family protein	-
GII31_RS19730 (GII31_19630)	4521343..4521690	-	348	WP_260840128.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GII31_RS19735 (GII31_19635)	4521678..4521911	-	234	WP_213245050.1	hypothetical protein	Antitoxin
GII31_RS19740 (GII31_19640)	4522013..4523143	-	1131	WP_213245051.1	acyl-CoA dehydrogenase family protein	-
GII31_RS19745 (GII31_19645)	4523152..4524339	-	1188	WP_213245052.1	acyl-CoA dehydrogenase family protein	-
GII31_RS19750 (GII31_19650)	4524462..4526537	+	2076	WP_246221966.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4515659..4516873	1214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12383.27 Da
• Isoelectric Point: 8.0236

>T142623 WP_260840128.1 NZ_CP045809:c4521690-4521343 [Gordonia pseudamarae]
VAVVERGDVWWVDFGEPLGSEPGYRRPALIVSSDRFNRSRIATVIVSAVTSNLRLAAAPGNVELAAGTAGLPKDCVVNVS
QTLVVDRARLTEPIGTLAFHIMRRVDDGLRLVFAV

Nucleotide

• Length: 348 bp

>T142623 NZ_CP060790:1215515-1216099 [Acidovorax monticola]
ATGGATGGTGCGCTGGTTGACGGCCGACTGAATTTTCTGCGTGCCTGTGAGCGGTTGAAGGATGTGCTGCGCAGCGGCCA
CACATCGACGGGACGGCGCGAGAGCACGGCGGAGCACAGTTGGAGGCTGGGCCTGATGGCAATGGTCTTCATGGACCAGT
TGGGTGACATCGACCGCGCCAGGGTGCTGGAGCTTTGCCTGGTGCACGACCTGGGCGAAGCGCTGGGAGGAGATGTGCCG
GCGCCCCAGCAGCCTGCGGGCGGCAGCAAGGACGCCACCGAGCGCCGTGATCTGCTGAAAATATGCGAGACGCTGGACCC
GGCGATGCGCGAGAAGATCGTCGGGCTGTGGGACGAGTATGCGCAGGCCGCCACGCCGGAAGCACGCGCCGTGAAAGCGC
TCGACAAACTCGAAACCATCCTGCAGCACACGCAGGGCGCCAATCCCCCGGGCTTCGACTACGCGTTCAATCTCGCCTAT
GGACAGAAGTACACGAAGGCCCTGCCGGTCCTCGAAGTGCTCCGAGGTCGGATAGACAATGCGACGCGTGCCCGCATGGC
CCGGCCGGACGGCTCACGGGAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8551.42 Da
• Isoelectric Point: 4.2264

>AT142623 WP_213245050.1 NZ_CP045809:c4521911-4521678 [Gordonia pseudamarae]
MKTAISIPDDVFREADSAANRLGWSRSQLYTRAVREFLERQEEDSVTAALNDIADGLNSDPVPAVGRSLIESGAWQW

Nucleotide

• Length: 234 bp

>AT142623 NZ_CP060790:1214820-1215410 [Acidovorax monticola]
ATGAAACAGATACCAACGGTCGATGCCGCTGTTTCGCCCGCTGCACGATCCGGGCTGCCCTACTCGCCGGGCGCGATGCT
GCGCCGCTGGCGCATGCTGCGTCGGATCAAGCAGGAGCATGCAGCGCAATTGTTCGGCGTGTCGCAATCGACCGTGTCCC
GCTGGGAGTCCGGCATCCAGTCGGTCTCTCGGGACGAACTGGTCCGGATCGAGGGATTCTTCGCCGCACGCCTGGACTCG
GCCGCCGACTACGCTCTGGAGCGCCTTGTGAGCCACAGCACGCAGCCGGTTCACCTCGTATGTGATCTCACCCATCGGCT
GCTTGCTTGTTCCTCGCCACGGGCGGCAGAGTTTGCCTCGCCGCTCCCGGAGTTGATGGGCCGGTCGCTATGGCCGTTTG
CCACCGCTGAAATCGTGGCACGCGAACAGTCGCTCCGCGACGCGGGGTGGCACGACCATGCCACGCCCTCCATCGAATTC
GACACCGGCTGCAACACGTCGGCGCAGGTGCCGATCCGCATGAGCCGCTGCCGATGGACGCGCCTGACCCTGTCGGACGG
CACGGTTGCACGCCTGGTAGAGACGCTTTGA