Detailed information of TA system TA142614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VapB
• Location: 2852618..2853234
• Replicon: chromosome
• Accession: NZ_CP045806
• Organism: Gordonia pseudamarae strain BEN371

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GII33_RS12555
• Protein ID: WP_213243471.1
• Coordinates: 2852830..2853234 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GII33_RS12550
• Protein ID: WP_213243469.1
• Coordinates: 2852618..2852833 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII33_RS12520 (GII33_12505)	2847690..2847962	-	273	WP_213243460.1	hypothetical protein	-
GII33_RS12525 (GII33_12510)	2848345..2849832	-	1488	WP_213243462.1	AAA family ATPase	-
GII33_RS12530 (GII33_12515)	2849930..2850160	-	231	WP_213243463.1	helix-turn-helix domain-containing protein	-
GII33_RS12535 (GII33_12520)	2850217..2850876	+	660	WP_213243465.1	hypothetical protein	-
GII33_RS12540 (GII33_12525)	2850873..2852246	+	1374	WP_213243467.1	site-specific integrase	-
GII33_RS12550 (GII33_12535)	2852618..2852833	+	216	WP_213243469.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GII33_RS12555 (GII33_12540)	2852830..2853234	+	405	WP_213243471.1	PIN domain nuclease	Toxin
GII33_RS12560 (GII33_12545)	2853288..2854727	-	1440	WP_213243473.1	ribosome biogenesis GTPase Der	-
GII33_RS12565 (GII33_12550)	2854724..2855455	-	732	WP_246221848.1	(d)CMP kinase	-
GII33_RS12570 (GII33_12555)	2855452..2856495	-	1044	WP_213243475.1	pseudouridine synthase	-
GII33_RS12575 (GII33_12560)	2856505..2857191	-	687	WP_213250272.1	SMC-Scp complex subunit ScpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-38)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14906.07 Da
• Isoelectric Point: 4.6805

>T142614 WP_213243471.1 NZ_CP045806:2852830-2853234 [Gordonia pseudamarae]
VILVDTSVWIEYLRDTDDPVVTELSSLIDSGADLRMTEPIAMELLAGADNPRRDALISQLTNSFPTLPVEPLVDYRAAAQ
IFASVRRIGHPLRSLNDCLIAAIAVRTSTILFERDRDFQFISKVTPLALHRNST

Nucleotide

• Length: 405 bp

>T142614 NZ_CP060783:4251726-4252301 [Diaphorobacter aerolatus]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8103.29 Da
• Isoelectric Point: 5.8214

>AT142614 WP_213243469.1 NZ_CP045806:2852618-2852833 [Gordonia pseudamarae]
MTRTNIDLDDDLVAEVIKRFNVSTKKEAVDLALRRLVGVPLTTQFLHELRGIGWKGDLDELRAAEDPWKGR

Nucleotide

• Length: 216 bp

>AT142614 NZ_CP060783:4251259-4251729 [Diaphorobacter aerolatus]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA